Project description:The aim of the project is to determine the expression profile of Arabidopsis DEFL during sexual reproduction, in particular seed development. To this aim, we generated a customised Affymetrics array that was hybridized with labeled cDNA synthetised from total RNA. Plant were grown in a control environment chamber and were pollinated manually to precisely generate different stages of seed development. Samples were collected in tripicates.
Project description:In this project, we developed a data resource based on the German winter oilseed rape cultivar Express 617. Plants were grown under controlled environmental conditions in the container-based system of the IPK PhenoSphere in 2020-2021. The dataset comprises gene expression data generated by RNA sequencing of different seed tissues across five stages of seed development, spanning early embryo/seed development, seed filling, and seed maturation. In the first stage (pre-storage), whole seeds were analysed. In the later four stages, developing seeds were dissected into four organs/tissues (SC = seed coat, IC = inner cotyledon, OC = outer cotyledon, and RA = radicle). This dataset (mRNA sequencing) is part of a series of multi-omics data generated in the frame of the AVATARS project.
Project description:We previously showed CHS siRNAs levels to be high in immature seed coats of seed with dominant alleles of the I locus that controls seed color (Tuteja et al., Plant Cell 2009; Cho et al., Plos One, 2013) and to be much lower in several other tissues sampled (Zabala et al., BMC Plant Biology 2012). Here, we examined 15 additional small RNA populations by high-throughput sequencing of developing seed coats of various stages from cultivars with the dominant I and i-i alleles that specify yellow seed coats or from spontaneous mutations to pigmented seed coats. The CHS siRNAs were tissue specific and found at high levels of up to 5700 normalized RPMs (reads per millions) in immature seed coats but were less than 6 RPMs in libraries from 21 non-seed coat samples including the embryonic axis, different stages of seedlings cotyledons, vegetative buds, and shoot tips, illustrating the tightly controlled tissue-specific expression of the CHS siRNAs in seed coats.
2025-07-31 | GSE239484 | GEO
Project description:Mycobiota of Coix seed samples
Project description:This project aims to investigate the effects of 3% oxygen environments on THP-1 cells as compared to 20% oxygen environments. THP-1 cells (pre-seed) were divided into 2 different conditions and cultured in 20% and 3% oxygen environments for 24h. Samples were performed in triplicates.
Project description:Longissimus muscle samples were collected from lambs exposed in utero to mycotoxins (E-, endophyte-free tall fescue seed without ergot alkaloids or E+, endophyte-infected tall fescue seed containing ergot alkaloids) during mid-gestation (MID; E+/E-; N) or late-gestation (LATE; E-/E+; T) harvested at two developmental stages (FETAL, gestational d133) or (MKT, near maturity, 250 d of age). Muscle samples were examined to determine the impact of in utero mycotoxin exposure on skeletal muscle fiber hypertrophy and the miRNA transcriptome at FETAL and MKT.