Project description:E. coli TG1 with pBS(Kan)Synhox can produce more hydrogen than TG1/pBS(Kan). To reveal the difference of metabolic activity (gene expression) between these strains in producing hydrogen, the differential gene expression analyses were performed. All samples cultured in complex medium with fructose containg 5 mM IPTG. Experiment Overall Design: Strains: E. coli TG1/pBS(Kan)Synhox and TG1/pBS(Kan) Experiment Overall Design: Medium: Complex with fructose Experiment Overall Design: Hydrogen producing cells Experiment Overall Design: Time: 6 hour