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OdHalVisc

ABSTRACT: Haliclona viscosa

PROVIDER: PRJEB86350 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	odHalVisc2.Bacteria_1.1.primary.fa.gz	Other
	odHalVisc2.Endozoicomonas_sp_1.1.primary.fa.gz	Other
	odHalVisc2.Gammaproteobacteria_2.1.primary.fa.gz	Other
	odHalVisc2.Gammaproteobacteria_3.1.primary.fa.gz	Other

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odHalVisc

Project description:Haliclona viscosa, genomic and transcriptomic data

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odHalVisc2.metagenome

Project description:Haliclona viscosa sponge metagenome assembly, odHalVisc2.metagenome

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Co-cultivation and transcriptome sequencing of two co-existing fish pathogens Moritella viscosa and Aliivibrio wodanis.

Project description:Aliivibrio wodanis and Moritella viscosa have often been isolated together from fish with winter ulcer. Little is known about the interaction between the two bacterial species and how the presence of one bacterial species affects the behaviour of the other. The impact on bacterial growth in co-culture was investigated in vitro, and the presence of A. wodanis has a strong inhibitorial effect on M. viscosa. Further, we have sequenced the complete genomes of these two marine Gram-negative species, and have performed transcriptome analysis of the bacterial gene expression levels from in vivo samples. Using bacterial implants in the fish abdomen, we demonstrate that the presence of A. wodanis is altering the gene expression levels of M. viscosa compared to when the bacteria are implanted separately. The impeding effect on growth and the change in the global gene expression pattern of M. viscosa when the two pathogens co-exists is discussed in this paper.

2014-11-30 | E-MTAB-2851 | biostudies-arrayexpress

Atlantic salmon scale explants in bacteria-host interaction studies: in vitro challenge model.

Project description:Atlantic salmon scale explants is a promising model for bacterial challenges. Exposure to pathogens (Moritella viscosa and Tenacibaculum finnmarkense) and commensal bacteria consistently upregulated a suite of immune genes in a dose response manner. This panel includes chemokines, cytokines, genes involved in eicosanoid metabolism, and both humoral and cellular effectors. The prevalence of genes related to signaling and communication suggests that salmon scales function as sentinels, mobilizing immune responses upon encountering bacteria. While common immune responses were evident across all trials, scales also revealed differences between bacterial species. Combining commensal bacteria with M. viscosa enhanced responses to pathogens, and differences were observed between M. viscosa and T. finnmarkense. The effects of commensal bacteria varied in magnitude. Exposed cells showed changes in morphology and increased iNOS expression.

2025-03-30 | GSE292873 | GEO

odHalCnid

Project description:Haliclona cnidata

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Project description:Haliclona cinerea

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Differential expression of miRNAs and mRNAs in Moritella viscosa challenge Atlantic salmon

Project description:Moritella viscosa is a bacterial pathogen causing winter-ulcer disease in Atlantic salmon. The lesions on affected fish lead to increased mortality, decreased fish welfare, and inferior meat quality in farmed salmon. MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional regulation by guiding the miRNA induced silencing complex to specific mRNA transcripts (target genes). The goal of this study was to identify miRNAs responding to Moritella viscosa in salmon by investigating miRNA expression in head-kidney and the muscle/skin from lesion sites caused by the pathogen. Protein coding gene expression was investigated by microarray analysis in the same materials.

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