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WT vs Nkx2.2-/- (null) cells at E15.5

ABSTRACT: WT vs Nkx2.2-/- (null) cells at E15.5

PROVIDER: PRJEB8642 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR768571.fastq.gz	Fastqsanger.gz
	ERR768572.fastq.gz	Fastqsanger.gz
	ERR768573.fastq.gz	Fastqsanger.gz
	ERR768574.fastq.gz	Fastqsanger.gz
	ERR768575.fastq.gz	Fastqsanger.gz

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WT vs Nkx2.2-/- (null) cells at E15.5

Project description:Whole transcriptome analysis of Ngn3-expressing cells in WT and Nkx2.2 Null mouse embryos.

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RNA-Seq analysis of E15.5 pancreas of both whole body Nkx2.2 mutant embryos and endocrine progenitor specific Nkx2.2 mutant embryos

Project description:Aim: Transcriptional analysis of E15.5 whole pancreas of Nkx2.2-LacZ/LacZ embryos versus control and Ngn3-Cre; Nkx2.2-flox/flox embryos versus control Methods: Embryonic pancreata were isolated at E15.5 from Nkx2.2 mutant mice and controls. Total RNA was extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq. Results: There is significant overlap between the differentially expressed genes of whole body Nkx2.2 mutant embryos and endocrine progenitor specific Nkx2.2 mutant embryos; many of the downregulated genes (p-value < 0.05) are genes involved in beta cell function. Conclusion: Nkx2.2 functions within the endocrine progenitor lineage to activate beta cell genes

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NKX2.2

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Functional analysis of the Nkx2.2 SD domain: gene expression profiling of E16.5 Nkx2.2 SD mutant pancreata versus wild-type littermate pancreata

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Project description:The goal of this experiment was to analyze expression changes in the pancreas at embryonic days 12.5 and 13.5 between wild type and Nkx2.2 null mice. We know that Nkx2.2 is essential for pancreatic endocrine differentiation and development. At these early time points which are critical for endocrine cell specification, we would like to identify a transcriptional program that Nkx2.2 regulates. We would also like to identify direct and functional transcriptional targets of Nkx2.2.

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