Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens

ABSTRACT: Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens

OTHER RELATED OMICS DATASETS IN: GSE7906

PROVIDER: PRJNA100081 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide small RNAs analysis of simian virus 40 infected cells

Project description:The goal of this study was to compare small RNA profiles (sRNA-seq) of simian virus 40 infection and mock infected Vero cells at 72 hours post infection.

2017-01-11 | GSE93350 | GEO

Genome-wide analysis of simian virus 40 infected cells

Project description:The goal of this study was to compare the whole transcriptional profile (RNA-seq) of simian virus 40 infection and mock infected Vero cells at 72 hours post infection. Circular RNAs (circRNAs), identified as a class of widely expressed endogenous regulatory RNAs, are involved in diverse physiological and pathological processes. However, their role in viral pathogenesis and cellular antiviral response remains unexplored. In this study, a potent DNA tumor virus, simian virus 40 (SV40), was used as a model to investigate the viral influences on cellular circRNA transcriptome. Using RNA-seq, 15,241 putative circRNAs were identified de novo from 5,057 parental genes in monkey kidney–derived Vero cells. The expression of selected circRNAs was confirmed by reverse transcription-polymerase chain reaction and Sanger sequencing. Further analysis showed that most circRNAs comprised multiple exons, and most parental genes produced multiple circRNA isoforms. A total of 134 significantly dysregulated circRNAs, including 103 upregulated and 31 downregulated circRNAs, were found after SV40 infection. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that various cancer-related pathways, including p53 and Wnt pathway, could be affected by SV40 infection via the alteration of the circRNA hosting genes. Moreover, diverse cellular immune pathways, including Toll-like receptor pathway and Janus kinase–signal transducer and activator of transcription pathway, could also be affected by SV40 infection. An integrated circRNA-miRNA-gene analysis suggested the putative function of circRNAs as cellular and viral miRNA decoys to indirectly regulate the gene expression during SV40 infection. This study presented the first comprehensive expression and functional profile of circRNAs in response to SV40 infection, thus providing new insights into the mechanisms of viral pathogenesis and cellular immune response.

2017-01-13 | GSE93555 | GEO

Simian immunodeficiency virus

Project description:Simian immunodeficiency virus overview

| PRJNA69627 | ENA

Simian immunodeficiency virus

Project description:Simian immunodeficiency virus Variation

| PRJNA176620 | ENA

Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens

Project description:SV40 transforms cells through the action of two oncoproteins, large T antigen and small t antigen. Small t antigen targets phosphatase PP2A, while large T antigen stimulates cell proliferation and survival by action on multiple proteins, including the tumor suppressors Rb and p53. Large T antigen also binds components of the transcription initiation complex and several transcription factors. We examined global gene expression in SV40-transformed mouse embryo fibroblasts, and in enterocytes obtained from transgenic mice. SV40 transformation alters the expression of approximately 800 cellular genes in both systems. Much of this regulation is observed in both MEFs and enterocytes and is consistent with T antigen action on the Rb-E2F pathway. However, the regulation of many genes is cell-type specific, suggesting that unique signaling pathways are activated in different cell types upon transformation, and that the consequences of SV40 transformation depends on the type of cell targeted.

2009-09-01 | GSE7906 | GEO

Simian immunodeficiency virus

Project description:Simian immunodeficiency virus Genome sequencing and assembly

| PRJNA317777 | ENA

Host response and dysfunction in the CNS during chronic simian immunodeficiency virus infection

2012-01-29 | GSE35397 | GEO

Host response and dysfunction in the CNS during chronic simian immunodeficiency virus infection

Project description:Molecular basis for CNS dysfunction in simian immunodeficiency virus-infected rhesus monkeys. We used microarrays to identify differentially expressed genes in chronic simian immunodeficiency virus-infected rhesus monkeys. Frontal lobe samples were obtained from control and SIV infected animals for RNA extraction and hybridization on Affymetrix microarrays. We sought to better understand the gene that changes in gene expression with SIV infection in the frontal lobe.

2012-01-29 | E-GEOD-35397 | biostudies-arrayexpress

Simian immunodeficiency virus strain:SIVmac251

Project description:Simian immunodeficiency virus strain:SIVmac251 Raw sequence reads

| PRJNA551921 | ENA

Simian immunodeficiency virus strain:SIVdeltaB670

Project description:Simian immunodeficiency virus strain:SIVdeltaB670 Genome sequencing and assembly

| PRJNA353138 | ENA