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Effect of 15-PGDH inhibition on skeletal muscle after sciatic nerve transection by single nuclei analysis

ABSTRACT: Effect of 15-PGDH inhibition on skeletal muscle after sciatic nerve transection by single nuclei analysis

PROVIDER: PRJNA1001056 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25486673.fastq.gz	Fastqsanger.gz
	SRR25486674.fastq.gz	Fastqsanger.gz
	SRR25486675.fastq.gz	Fastqsanger.gz
	SRR25486676.fastq.gz	Fastqsanger.gz
	SRR25486677.fastq.gz	Fastqsanger.gz

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Effect of 15-PGDH inhibition on skeletal muscle after sciatic nerve transection by single nuclei analysis

Project description:There are currently no approved treatments for the weakness and paralysis that result from loss of peripheral nerve function with aging, trauma, or neuromuscular disease. Here we use single nuclei sequencing to show that denervation due to transection of the sciatic nerve triggers a striking increase in the prostaglandin degrading enzyme 15-hydroxyprostaglandin dehydrogenase (15-PGDH) in skeletal muscle.

2023-10-23 | GSE239848 | GEO

MicroRNA profiling in dorsal root ganglion after sciatic nerve injury

Project description:Changes in microRNA (miRNA) expression in the mouse L4 and L5 dorsal root ganglion following unilateral sciatic nerve transection. The timepoint of 7 days post-axotomy was chosen to capture miRNA expression profiles at a time when the injured neurons were beginning to regenerate. Two condition experiment, paired control DRG vs axotomised DRG following unilateral sciatic nerve transection. 3 biological replicates, one replicate per array. Dye swap in Replicate 2.

2011-05-06 | E-GEOD-29096 | biostudies-arrayexpress

Enhancer Analysis in Peripheral Nerve: ChIP-seq of H3K27ac in P15 sciatic nerve

Project description:ChIP-seq of H3K27ac in P15 sciatic nerve was used to identify actively engaged enhancers in Schwann cells, which constitute the majority of nuclei in peripheral nerve.

2015-04-01 | GSE64971 | GEO

FACS purified Nav1.8 lineage sensory neuron whole transcriptome changes following peripheral inflammation (CFA) or sciatic nerve injury (SNI).

Project description:The goal of this study was to analyze global gene expression in FACS purified Nav1.8 lineage sensory neurons, which include nociceptor neurons that detect damaging/noxious stimuli, following peripheral inflammation by intraplantar injection of Complete Freund's Adjuvant (CFA) or Sciatic Nerve Injury (SNI) by nerve transection. Nav1.8 Trangsgenic TdTomato+ neurons were purified from Lumbar L4-L6 dorsal root ganglia (DRG) by flow cytometry from mice on the ipsilateral or contralateral sides, following Complete Freund's Adjuvant injection (day 1) or sciatic nerve transection (day 5). Neurons were then analyzed for transcriptional gene expression by microarray analysis.

2023-01-30 | GSE221834 | GEO

MicroRNA profiling in dorsal root ganglion after sciatic nerve injury

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Gene expression from aged and young sciatic nerve after nerve injury

Project description:Sciatic nerve ligation was performed on cohorts of 2-month and 24-month old animals. Resulting gene-expression data were generated from sciatic nerve 1 and 4 days after injury compared to naïve animals. Results show differences in sciatic nerve responses with normal aging. Total RNA taken from sciatic nerves from 2-month and 24-month old animals at either day 0, 1 and 4 after sciatic nerve crush injury.

2014-07-30 | E-GEOD-59040 | biostudies-arrayexpress

Rat sciatic nerve axoplasm proteome is enriched with ribosomal proteins during regeneration processes.

Project description:Analysis of extruded axoplasm proteomes from adult rat sciatic nerves in vivo by Label Free Quantitative Proteomics (LFQ) and comparison of normal and injury (18 hours post complete nerve transection) conditions. A combination of BONCAT and MS/MS were also performed to analyze the top axonal newly synthetized proteins in vivo.

2020-11-18 | MSV000086475 | MassIVE

Single cell RNA-seq analysis of sensory neurons reveal diverse injury responses after sciatic nerve transection

Project description:We reported the gene expression analysis of different types of sensory neuron with peripheral nerve transection treatment on single cell level. We found substantial variation between myelinated large diameter neurons and small diameter nonpeptidergic nociceptors, in both terms of regeneration response genes regulation as well as fraction of cells respond to nerve injury.

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Gene expression from aged and young sciatic nerve after nerve injury

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Clathrin IP_mouse sciatic nerve axons_Roylab_UCSD

Project description:Sciatic nerve axon segments from adult mouse were isolated. Following enzymatic digestion, lysate was subjected to incubation with beads conjugated with either clathrin heavy chain antibody or control IgG antibody for clathrin immunoprecipitation. MudPIT analysis was subsequently performed to identify proteins co-precipitating with clathrin.

2021-08-12 | PXD027857 | Pride

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