Project description:These experiments were designed to determine the usefulness of the spurge/cassava euphorbiaceae arrays for investigating gene expression in other euphorbs. Keywords: heterologous hybridization euphorbia
Project description:This study is a time course of growth induction (0, 12, 24, 48, and 72 hrs) following the breaking of paradormancy in underground buds of the perennial weed leafy spurge (Euphorbia esula). Keywords: Time course, growth induction, apical dominance, paradormancy, root buds, leafy spurge
Project description:Transcriptome changes were investigated for Euphorbia esula (leafy spurge) seeds with a focus on the effect of constant and diurnal fluctuating temperature on dormancy and germination. Leafy spurge seeds do not germinate when incubated for 21 days at 20°C constant temperatures, but nearly 30% germinate after 21 days under fluctuating temperatures 20:30°C (16:8 h). Incubation at 20°C for 21 followed by 20:30°C resulted in approximately 63% germination in about 10 days. A cDNA microarray representing approximately 22,000 unique sequences was used to profile transcriptome changes.
