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A comparison between GalT-/- hCD39 hCD55 and GalT-/- hCD39 hCD46 hCD55 TBM pig kidney transplanted in non-human primates

ABSTRACT: A comparison between GalT-/- hCD39 hCD55 and GalT-/- hCD39 hCD46 hCD55 TBM pig kidney transplanted in non-human primates

PROVIDER: PRJNA1001921 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25508313.fastq.gz	Fastqsanger.gz
	SRR25508314.fastq.gz	Fastqsanger.gz
	SRR25508315.fastq.gz	Fastqsanger.gz
	SRR25508316.fastq.gz	Fastqsanger.gz

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A comparison between GalT-/-;hCD39;hCD55 and GalT-/-;hCD39;hCD46;hCD55;TBM pig kidney transplanted in non-human primates

Project description:Due to shortage of donor kidney, genetically modified kidney xenograft from pigs is considered. To select the optimal gene modification for xenotransplantation, knockout of carbohydrate genes or knockin of protective proteins have been applied. In this study, we utilized GalT- /-;hCD39;hCD55 and GalT-/-;hCD39;hCD46;hCD55;thrombomodulin (TBM) pigs for transplantation in non-human primates. NHPs survived for 4 weeks after kidney transplantation (4 WAT) from GalT-/-;hCD39;hCD55 pig and 6 WAT from GalT- /-;hCD39;hCD46;hCD55;TBM pig. However, mRNA sequencing and immunohistochemistry analysis revealed that 6 WAT kidney showed the severe apoptosis, inflammation, loss of renal function, and renal fibrosis than 4 WAT kidney. These results suggest that additional knockin of anti-complement (hCD26) and anti-coagulation (TBM) is not sufficient to inhibit the renal damage.

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