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Effect of roots subjected to 300 mg/l PS-COOH treatments and to control conditions for RNA-Seq in cotton

ABSTRACT: Effect of roots subjected to 300 mg/l PS-COOH treatments and to control conditions for RNA-Seq in cotton

PROVIDER: PRJNA1001952 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25548170_1.fastq.gz	Fastqsanger.gz
	SRR25548170_2.fastq.gz	Fastqsanger.gz
	SRR25548171_1.fastq.gz	Fastqsanger.gz
	SRR25548171_2.fastq.gz	Fastqsanger.gz
	SRR25548172_1.fastq.gz	Fastqsanger.gz

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Effect of roots subjected to 300 mg/l PS-COOH treatments and to control conditions for RNA-Seq in cotton

Project description:Roots from 10 independent plants subjected to 300 mg/l PS-COOH treatments and to control conditions were pooled separately to form single biological replicates for RNA-seq analysis. Transcriptomics analyses showed that antioxidant activity-related genes were significantly upregulated after PS-COOH treatments, indicating that oxidative stress was induced in cotton.

2024-01-15 | GSE240032 | GEO

Azospira oryzae PS strain:PS

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Project description:The modes of triazole reproductive toxicity have been characterized by an observed increased in serum testosterone and reduced insemination and fertility indices. The key events involved in the disruption in testosterone homeostasis and reduced fertility remain unclear. Gene expression analysis was conducted on liver from Sprague Dawley rats dosed with myclobutanil (300 mg/kg/day), propiconazole (300 mg/kg/day), or triadimefon (175 mg/kg/day) for 72 hours. Pathway-based analysis highlighted key biological processes affected by all three triazoles in the liver including fatty acid catabolism, steroid metabolism, and xenobiotic metabolism. Within the pathways identified in the liver, specific genes involved in phase I-III metabolism and fatty acid metabolism were affected by all three triazoles. These modulated genes are part of a network of lipid and testosterone homeostasis pathways regulated by the constitutive androstane (CAR) and pregnane X (PXR) receptors. Gene expression profiles from this study indicate triazoles activate CAR and PXR; increase fatty acid catabolism and steroid metabolism in the liver; constituting a plausible series of key events contributing to the observed disruption in testosterone homeostasis. Experiment Overall Design: A total of 12 liver samples were analyzed. Three biological replicates each for the controls, 300 mg/kg/day myclobutanil, 300 mg/kg/day propiconazole, and 175 mg/kg/day triadimefon.

2008-04-08 | E-GEOD-10409 | biostudies-arrayexpress

Sphaerochaeta sp. PS strain:PS

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Escherichia coli PS strain:PS

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RNAseq analysis of PS+ vs PS- non-naive CD8 T cells sorted from peripheral blood of COVID-19 patients

Project description:We report the transcriptional profile of phosphatidylserine (PS) positive and PS negative non-naive peripheral blood CD8 T cells from patients infected with SARS-CoV-2. PS+ CD8 T cells showed increased expression of cell cyclce and cell division associated genes and reduced expression of genes regulating translational initiation, when compared to PS- CD 8 T cells. Furthermore, PS+ CD8 T cells show increased expression of effector T cell genes.

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Bradyrhizobium japonicum strain:USDA 300 | isolate:USDA 300

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RNAseq analysis of PS+ vs. PS- effector CD8 T cells sorted from spleen of LCMV-Armstrong infected mice

Project description:We report the transcriptional profile of phosphatidylserine (PS) positive and PS negative CD44+CD62L- effector CD8+ T cells from LCMV-Armstrong infected mice sorted from spleens on day5 amd day10 after infection. PS was stained in vivo by injection of MFG-E8-eGFP. PS+ cells acquire PS through binding of extracellular vesicles (EVs). Binding of EVs by effector CD8+ T cells is strongly enhanced during LCMV infection and peaks between day5 and day10 post infection. Effector T cell gene signature is increased in EV+ CD8+ T cells compared to their EV- counterparts.

2023-03-29 | GSE201507 | GEO

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