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A novel viral tool designed with enhancer-driven gene expression (EDGE) technology allows specific labelling of cell types in wild type rodents

ABSTRACT: A novel viral tool designed with enhancer-driven gene expression (EDGE) technology allows specific labelling of cell types in wild type rodents

PROVIDER: PRJNA1001963 | ENA |

REPOSITORIES: ENA

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A novel viral tool designed with enhancer-driven gene expression (EDGE) technology allows specific labelling of cell types in wild type rodents

Project description:The mammalian brain contains an unimaginable number of neurons, who are all intricately connected to form complex neuronal circuits. Neurons that exhibit similar properties are often defined as belonging to the same cell-type, which can be determined for example by analysing the genetic profile of the cells. A key aspect of studying brain function on a circuit level is the ability to target specific cell types, but this necessitates sophisticated molecular and genetic tools. Here, we contribute to the ever-expanding toolkit of genetic targeting approaches, by utilizing Enhancer-Driven Gene Expression (EDGE) technology to create viral tools that allow specific targeting of cell populations in the hippocampus and parahippocampus of wild type mice. We also demonstrate the applicability of our tool in wild type rats, emphasising the versatility of this technology in cross-species studies. Cell-type specific targeting in wild type animals is something that could be of great benefit to the field, also in other species than mice where transgenic lines are not common.

2023-09-14 | GSE240042 | GEO

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Crosstalk with astrocytes establishes tumor edge in glioblastoma

Project description:Clinical outcomes for patients with glioblastoma (GBM) are extremely poor due to inevitable tumor recurrence even after extensive treatments. These recurrences are thought to manifest from cells located within the tumor edge. Despite this, the precise molecular mechanism governing GBM spatial phenotypic heterogeneity (e.g. edge vs. core) and subsequent tumor recurrence remains poorly elucidated. Here, using patient-derived GBM core and edge tissues, we analyzed transcriptional and metabolic signatures in an effort to determine how GBM facilitates the edge phenotype and its associated recurrence-initiating cells (RICs). In so doing, we unexpectedly identified CD38 as an essential protein in the formation of the edge phenotype and found a CD38-driven interaction between edge GBM cells and neighboring astrocytes that communally develops a GBM edge that is unresectable by surgery and retains RICs.

2021-12-25 | GSE135408 | GEO

Rodents

Project description:Viral metagenomic in in rodents in urban and rural locations in Tanzania

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Gene Expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice

Project description:Gene Expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice Affymetrix GeneChipÂ® Mouse Genome 430 array was used to study the gene expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice Gene Expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice

2016-07-21 | E-GEOD-73229 | biostudies-arrayexpress

Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy

Project description:Intratumor spatial heterogeneity facilitates therapeutic resistance in glioblastoma (GBM). Nonetheless, understanding of GBM is limited to the resectable tumor core lesion while the seeds for recurrence reside in the unresectable tumor edge. In this study, stratification of GBM to core and edge demonstrated clinically relevant surgical sequelae. We establish regionally derived models of GBM edge and core that retain their spatial identity in a cell autonomous manner. Edge cells show a higher capacity for infiltrative growth, while core cells demonstrate greater therapy resistance. Investigation of intercellular signaling between these two cell populations uncovered the paracrine crosstalk from tumor core that provokes malignancy and therapy resistance of edge cells. These phenotypic alterations were initiated by HDAC1 in GBM core cells which subsequently affect edge cells by secreting the soluble form of CD109 protein. Our data reveal the role of intracellular communication between regionally different populations of GBM cells in tumor recurrence.

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Labelling transcriptional response to dissociation using SLAMseq

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iTag labelling of Liver, Plasma and VsWAT RNA

Project description:Using a novel method for metabolic labelling of RNA called iTag RNA we isolate hepatocytes' and kidney proximal epithelial cells' transcriptional response to a dietary challenge and identify multiple hepatocyte-secreted small ccfRNAs in plasma, and transfer of these hepatic derived ccfRNAs to VsWAT.

2020-03-02 | GSE143562 | GEO

iTag labelling of Liver, Plasma and VsWAT RNA

2022-06-27 | GSE133458 | GEO

Gene Expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice

Project description:Gene Expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice Affymetrix GeneChip® Mouse Genome 430 array was used to study the gene expression in d5 wound-edge tissues of MFG-E8 WT and MFG-E8 KO mice

2016-07-21 | GSE73229 | GEO

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