Project description:Analysis of function of CD11c+ cells from middle-aged and young mice at gene level. This experiment provided insight into the different genes that plays roles in inflammation, immune response and mainly arachidonic acid cascade that are differentiall expressed in CD11c+ cells from middle aged and young mice. Total RNA was isolated from pulmonary CD11c cells (separated using magnetic beads) from middle-aged and young mice

2015-08-11 | E-GEOD-71868 | biostudies-arrayexpress

Hippocampal circadian proteomes of young and middle-aged mice

Project description:Hippocampal tissues from young and middle-aged C57BL/6J mice were harvested at 4-hour intervals over two days and processed for proteomic analysis using label-free quantification.

2020-05-26 | PXD013364 | Pride

Single cell multiome profiles of bone marrow hematopoietic stem and progenitor cells from young and aged donors.

Project description:To investigate molecular differences between HSPCs from young and aged donors, we performed 10x Single Cell Multiome on bone marrow HSPCs from Young, Middle Aged, and Older Aged donors.

2024-06-24 | GSE235646 | GEO

Single cell transcriptomics analysis of young Ncstnfl/fl (control), middle-aged Ncstnfl/fl (control) and middle-aged LeprCre; Ncstnfl/fl (cKO) bone and bone marrow

Project description:Skeletal stem and progenitor cells (SSPCs) perform bone maintenance and repair. With age, they produce fewer osteoblasts and more adipocytes leading to a loss of skeletal integrity. The molecular mechanisms that underlie this detrimental transformation are largely unknown. Single-cell RNA sequencing revealed that Notch signaling becomes elevated in SSPCs during aging. To examine the role of increased Notch activity, we deleted Nicastrin, an essential Notch pathway component, in SSPCs in vivo. Middle-aged conditional knockout mice displayed elevated SSPC osteo-lineage gene expression, increased trabecular bone mass, reduced bone marrow adiposity, and enhanced bone repair. Thus, Notch regulates SSPC cell fate decisions, and moderating Notch signaling ameliorates the skeletal aging phenotype, increasing bone mass even beyond that of young mice. Finally, we identified the transcription factor Ebf3 as a downstream mediator of Notch signaling in SSPCs that is dysregulated with aging, highlighting it as a promising therapeutic target to rejuvenate the aged skeleton.

2023-08-18 | GSE240291 | GEO

Gene expression pattern of pulmonary CD11c+ cells from middle-aged and young mice.

2015-08-11 | GSE71868 | GEO

Hippocampal transcript profile in young and middle-aged mice

Project description:We carried out a global survey of age-related changes in mRNA levels in the C57BL/6NIA mouse hippocampus and found a difference in the hippocampal gene expression profile between 2-month-old young mice and 15-month-old middle-aged mice correlated with an age-related cognitive deficit in hippocampal-based explicit memory formation. Middle-aged mice displayed a mild but specific deficit in spatial memory in the Morris water maze. Keywords: age comparison

2006-06-20 | GSE5078 | GEO

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