Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon

ABSTRACT: Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon

PROVIDER: PRJNA1003432 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25569900_1.fastq.gz	Fastqsanger.gz
	SRR25569900_2.fastq.gz	Fastqsanger.gz
	SRR25569901_1.fastq.gz	Fastqsanger.gz
	SRR25569901_2.fastq.gz	Fastqsanger.gz
	SRR25569902_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 60

Similar Datasets

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon [scrna-seq]

Project description:Most organs have tissue resident immune cells that function during tissue development, homeostasis and disease. Human organoids, derived either from adult tissues or from pluripotent stem cells (PSCs), have been lacking these immune cells, which limits their utility to model many normal and disease processes. Here we describe that human PSC-derived colonic organoids (HCOs) co-develop a diverse population of immune cells. Comparisons to the developing human hematopoietic cells, demonstrate that HCO cultures developed hemogenic endothelium and erythromyeloid progenitors that undergo stereotypical steps in differentiation, resulting in the generation of macrophages. HCO macrophages acquired a transcriptional signature most similar to human fetal small and large intestine tissue-resident macrophages. Functional analyses demonstrate that HCO-macrophages modulate cytokine secretion in response to pro- and anti-inflammatory signals. In addition, macrophages were able to phagocytose and mount a robust response to pathogenic bacteria. In HCOs that were transplanted into mice, macrophages expanded within the colonic organoid tissue, established a close association with the colonic epithelium and were not displaced by the host bone marrow-derived macrophages. These studies support the conclusion that hemogenic endothelium in HCOs gives rise to multipotent hematopoietic progenitors and functional tissue-resident macrophages.

2023-10-31 | GSE240358 | GEO

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon [bulk rna-seq]

2023-10-31 | GSE240356 | GEO

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon [scrna-seq]

Project description:Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon [scrna-seq]

| PRJNA1003439 | ENA

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon [bulk rna-seq]

Project description:Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon [bulk rna-seq]

| PRJNA1003423 | ENA

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon.

Project description:Most organs have tissue-resident immune cells. Human organoids lack these immune cells, which limits their utility in modeling many normal and disease processes. Here, we describe that pluripotent stem cell-derived human colonic organoids (HCOs) co-develop a diverse population of immune cells, including hemogenic endothelium (HE)-like cells and erythromyeloid progenitors that undergo stereotypical steps in differentiation, resulting in the generation of functional macrophages. HCO macrophages acquired a transcriptional signature resembling human fetal small and large intestine tissue-resident macrophages. HCO macrophages modulate cytokine secretion in response to pro- and anti-inflammatory signals and were able to phagocytose and mount a robust response to pathogenic bacteria. When transplanted into mice, HCO macrophages were maintained within the colonic organoid tissue, established a close association with the colonic epithelium, and were not displaced by the host bone-marrow-derived macrophages. These studies suggest that HE in HCOs gives rise to multipotent hematopoietic progenitors and functional tissue-resident macrophages.

| S-EPMC10913028 | biostudies-literature

Bulk RNA-seq of CRISPR SATB2 Knockout Human Colonic Organoids

Project description:To evaluate SATB2 function in human colonic epithelium, we knocked out the SATB2 gene in 3D cultured human colonic organoid.

2021-10-05 | GSE167285 | GEO

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-10-31 | GSE240363 | GEO

Transcriptomics of cytokine-treated human colonic epithelial organoids

Project description:Mapping of transcriptional changes elicited by cytokines mediating canonical immune responses in human colonic organoids

2022-08-08 | GSE190634 | GEO

VDR ChIP-Seq on colonic human organoids

Project description:VDR ChIP-Seq on colonic human organoids