Project description:The goal is to find out the gene expression changes when Dicer was inactivated in the murine heart. Keywords: Dicer mutant Dicer gene was specifically inactivated in the mouse heart using cardiac specific Nkx2.5 Cre knock-in line. Embryonic day 11.5 heart from mutant and control hearts were compared in triplicates.
Project description:We used Illumina Small RNA and RNA-Seq kits to prepare both small RNA and RNA-Seq libraries from total RNA isolated from either leptotenze/zygotene or pachytene spermatocytes purified from either Dgcr8 or Dicer germline conditional knockout mice. Conditional knockout mice were generated by using a Ddx4 promoter to drive cre excision of either Dgcr8 or Dicer at embryonic day 18. Mixed leptotene/zygotene or pachytene spermatocytes were then isolated from the testis of adult conditional knockout mice, along with paired WT littermates as a control. RNA was isolated from these spermatocytes using Trizol. Small RNA or RNA-Seq libraries were then prepped using Illumina's sequencing library preparation kits.
Project description:Transcriptional profiling of mouse comparing in vitro-derived DC progenitors from control and Gata2 conditional knockout mice. Two-condition experiment, Control DCs vs. G2 Knockout DCs. Biological replicates: 4 control, 3 Gata2 knockout, independently grown and harvested. One replicate per array.
Project description:Dicer knockout cause lipid metabolism dysregulation in small intestine. Analyses of transcriptomics and proteomics reveal key enzymes affected by Dicer knockout. Conditional knockout of Dicer in adult mice
Project description:Dicer knockout cause lipid metabolism dysregulation in small intestine. Analyses of transcriptomics and proteomics reveal key enzymes affected by Dicer knockout. Conditional knockout of Dicer in adult mice
Project description:To determine the temporal variation of mRNA levels, we collected and sequenced poly-adenylated RNA from all cell extracts, cytoplasmic and nuclear fractions of a conditional Dicer mutant [DTCM23/49 XY (Nesterova et al. 2008)] mouse Embryonic Stem Cells before induction of Dicer excision (day 0) and at days 4, 8, 10 and 12 following Dicer loss of function. coverage.