Project description:Our analysis provides a comprehensive picture of how P. trichocarpa responds to drought stress at physiological and transcriptome levels which may help to understand molecular mechanisms associated with drought response and could be useful for genetic engineering of woody plants. Drought stress treatment was performed dividing P. trichocarpa plants into the well-watered (WW) group (soil volumetric water content of 40â45 %) and the water-limited group (soil volumetric water content of 10â15 %). Two cDNA libraries constructed separately from the WW and WL groups were subjected to high-throughput Illumina sequencing.
Project description:The implementation of a cost-effective lignocellulosic ethanol production requires developing efficient high gravity processes (i.e. working at high substrate concentrations). During the fermentation processes, the presence of high concentration of insoluble solids leads to lower glucose consumption rates, reduced ethanol volumetric productivities, and the accumulation of intracellular reactive oxygen species (ROS). Major repressed biological processes include cell cycle progression, trehalose and glycogen biosynthesis, DNA repair mechanisms, and certain genes involved in the general cell stress response. On the other hand, genes related to the glutathione, thioredoxin and methionine scavenging systems are induced.
Project description:We identified the DNA binding sites of DPY-27 in C. elegans. DPY-27 was C-terminally tagged with GFP and the expression pattern was examined through fluorescent microscopy. The binding sites were determined using chromatin immunoprecipitation with anti-GFP antibody followed by illumina high-throughput sequencing (ChIP-seq). For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Identification of DPY-27 binding sites at embryonic stage
