Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Inhibition of SQLE or FDFT1 in RBE cells by shRNA, or RBE cells treated with lycorine hydrochloride

ABSTRACT: Inhibition of SQLE or FDFT1 in RBE cells by shRNA, or RBE cells treated with lycorine hydrochloride

PROVIDER: PRJNA1005187 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25629436_1.fastq.gz	Fastqsanger.gz
	SRR25629436_2.fastq.gz	Fastqsanger.gz
	SRR25629437_1.fastq.gz	Fastqsanger.gz
	SRR25629437_2.fastq.gz	Fastqsanger.gz
	SRR25629438_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Inhibition of SQLE or FDFT1 in RBE cells by shRNA, or RBE cells treated with lycorine hydrochloride

Project description:High-throughput sequencing was performed to analyze gene expression profiles of RBE cells after the the inhibition of SQLE or FDFT1 by lentiviruses-mediated shRNA, or RBE cells treated with lycorine hydrochloride.

2023-08-18 | GSE240746 | GEO

p53 transcriptionally regulates SQLE to modulate cholesterol synthesis and tumor growth

Project description:Cholesterol is essential for membrane biogenesis, cell proliferation and differentiation. The role of cholesterol in cancer development and the regulation of cholesterol synthesis are still under active investigation. Here we show that under normal-sterol conditions，p53 directly represses the expression of SQLE, a rate-limiting and the first oxygenation enzyme in cholesterol synthesis，in a SREBP2-independent manner. Through transcriptional downregulation of SQLE, p53 represses cholesterol production in vivo and in vitro, leading to tumor growth suppression. Inhibition of SQLE using small interfering RNA (siRNA) or terbinafine (a SQLE inhibitor) reverses the increased cell proliferation caused by p53 deficiency. Conversely, SQLE overexpression or cholesterol addition promotes cell proliferation, particularly in p53 wild-type cells. More importantly, pharmacological inhibition or shRNA-mediated silencing of SQLE restricts nonalcoholic fatty liver disease (NAFLD) -induced liver tumorigenesis in p53 knockout mice. Therefore, our findings reveal a role for p53 in regulating SQLE and cholesterol biosynthesis, and further demonstrate that downregulation of SQLE is critical for p53-mediated tumor suppression.

2021-07-18 | GSE176112 | GEO

Effect of depletion of SQLE on gene expression of B16F10 Melanoma cells in vivo

Project description:To investigate the function SQLE in the regulation of tumor immune microenvironment, we established B16F10 cell lines in which target gene has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells at same time points.

2023-01-01 | GSE216265 | GEO

RNA sequencing to determine gene expression changes in SQLE knockdown osteosarcoma cells

Project description:In our study, we found that SQLE was critical to osteosarcoma progression. To identify the mechanism of SQLE in osteosarcoma, we conducted the RNA-seq analysis of SQLE knockdown in osteosarcoma cell line U2OS cells.

2024-12-16 | GSE245562 | GEO

Gene expression profiling of RKO cells with or without SQLE knockdown.

Project description:RKO cells (WT or SQLE knockdown) were harvested and mRNA were extracted for high-throughput sequencing.

2024-05-20 | GSE232678 | GEO

ACC-MESO1 cells: Control shRNA vs USP22 shRNA

Project description:ACC-MESO1 cells: Control shRNA vs USP22 shRNA

| PRJNA414177 | ENA

Expression data from human Bladder cancer cell line J82 with control or overexpression of SQLE

Project description:Several reports showed that SQLE was upregulated in some types of cancer, and it is essential for cancer development. Herein, to uncover the role of SQLE in BCa, we performed the RNA-seq to detect the gene expression levels in BCa cell line J82 with overexpression of SQLE.

2024-04-28 | GSE231045 | GEO

E14 cells knockdown with shRNA

Project description:E14 cells knockdown with shRNA

| PRJNA972667 | ENA

Anylasis of the diatom Phaeodactylum tricornutum under 64-75-5,Tetracycline hydrochloride stress

Project description:Anylasis of the diatom Phaeodactylum tricornutum under 64-75-5,Tetracycline hydrochloride stress

2023-11-10 | GSE218473 | GEO

Gene expression profiling of RKO cells with or without SQLE knockdown.

Project description:Gene expression profiling of RKO cells with or without SQLE knockdown.

| PRJNA973571 | ENA