Project description:The goal of this study was to identify chromatin regulatory sites by FAIRE-seq under conditions of basal and increased dosage of transcription factor SF-1 in the H295R human adrenocortical tumor cell line. 4 samples: input DNA in basal SF-1 expression conditions - FAIRE-seq in basal SF-1 expression conditions - input DNA in SF-1 overexpression conditions - FAIRE-seq in SF-1 overexpression conditions
Project description:Ad4BP/SF-1 is a transcription factor regulating gene expression related to steroidogenesis and energy metabolism. Previous studies have indicated that Ad4BP/SF-1 regulates fetal Leydig cell differentiation from progenitor cells. However, the underlying regulatory mechanisms remain unclear. Here, we reveal that Ad4BP/SF-1 directly regulates energy metabolisms in differentiated fetal Leydig cells through transcriptional upregulation.
Project description:The goal of this study was to identify genomic binding sites of the NRSF/REST transcription factor under conditions of basal and increased SF-1 dosage in the H295R human adrenocortical tumor cell line. 4 samples: input DNA (2 replicates) - NRSF/REST ChIP basal SF-1 dosage - NRSF/REST ChIP increased SF-1 dosage
Project description:We describe here the transcripts induced after infection of zebrafish with viral haemorrhagic septicemia rhabdovirus (VHSV). Two days after infection, differentially expressed transcript levels from selected immune-related zebrafish genes were studied in both fins and internal organs (pooled spleen, head kidney and liver).