Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The combination of agrin, BRP44, and insulin (ABI) promote human CM proliferation

ABSTRACT: The combination of agrin, BRP44, and insulin (ABI) promote human CM proliferation

PROVIDER: PRJNA1006005 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25657271_1.fastq.gz	Fastqsanger.gz
	SRR25657271_2.fastq.gz	Fastqsanger.gz
	SRR25657272_1.fastq.gz	Fastqsanger.gz
	SRR25657272_2.fastq.gz	Fastqsanger.gz
	SRR25657273_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

The combination of agrin, BRP44, and insulin (ABI) promote human CM proliferation

Project description:Human cardiomyocytes (CMs) differ from those of rodents in many aspects, and methods that effectively improve cardiac performance in rodents have been unsuccessful in humans, indicating a translational gap between humans and rodents in cardiac regeneration. The CM proliferation transition between the neonatal and adult stages is well-established in humans and rodents. The aim of the study is to analyze human atrial CM proliferation-transition for narrowing down the translational gap between humans and rodents in cardiac regeneration.At first we extracted decellularized samples (DS) from human atrial specimens collected during routine congenital cardiac surgery from children diagnosed with ventricular septal defect (VSD). The DS was next used for cell culture and proteomic analyses. The proteins (such as Agrin, BRP44, and insulin) identified in the proteomic analysis were further verified by testing their ability to promote CM proliferation. The results showed that the combination of agrin, BRP44, and insulin (ABI) enhanced the effect of agrin.To further confirmed the role of ABI on human CM proliferation, we performed RNA-seq on the ABI-treated HiPSC-CMs. The results showed that ABI induced abundant enrichment of cell-cycle associated genes, which highlighting the synergy of hormones and anti-oxidation/metabolism in human CM proliferation.

2024-08-16 | GSE240986 | GEO

A. baumannii Cells: 1/2MIC chloramphenicol (Cm) tretment vs. 1/2MIC 4-hydroxybenzaldehyde (HBA) treatment vs. 1/4MIC combination of Cm and HBA treatment vs. Ethanol treatment

Project description:Transcriptional profiling of Acinetobacter baumannii ATCC17978 cells comparing each treated 1/2MIC Cm, 1/2MIC HBA and combination of 1/4MIC Cm and HBA cells with same volume of ethanol treated. Based on the gene expression, we analyzed results to confirm the relation between Cm and HBA to antibacterial synergism against A. baumannii. (Concentrations treated respectivly in all samples were adjusted to add same volume of Cm, HBA and combination)

2015-03-24 | GSE67173 | GEO

Gene expression profile of LAPC4 stimulated by 5α-Abi

Project description:LAPC4 cells were starved for 2 days and stimulated with 1µM 5α-Abi or 0.1nM DHT. Gene expression profiles are detected to determine the effect of 5a-Abi on prostate cancer cell line.

2016-03-01 | GSE75387 | GEO

Oscillospiraceae bacterium CM strain:CM

Project description:Chloromethanomonas sp. CM strain:CM Genome sequencing

| PRJNA678572 | ENA

Expression profiling of Abelson interactor-1 (Abi-1) deficient hematopoietic progenitor cells.

Project description:Gene expression profile of LSK-enriched population of hematopoietic progenitor cells from Abi-1 KO mice indicates activation of the NFκB pathway. In this dataset, we include the expression data obtained from Lineage-, Sca-1+, cKit+ (LSK)-enriched population of hematopoietic progenitor cells isolated from the bone marow of Abi-1 KO and WT animals. Abi1(fl/fl);Tg (Mx1- cre(-)) or Abi1(fl/fl);Tg (Mx1-cre(+)) mice were subjected to polyinosinic:polycytidylic acid [poly(I:C)]-induced activation of the Cre recombinase under control of the Mx1 promoter to obtain animals with an Abi1(fl/fl);Tg (Mx1-cre(-)) (Abi-1 WT) or Abi1(-/-);Tg (Mx1-cre(+)) (Abi-1 KO) genotype.

2016-06-14 | GSE83288 | GEO

Gene expression profile of LAPC4 stimulated by 5Î±-Abi

Project description:LAPC4 cells were starved for 2 days and stimulated with 1ÂµM 5Î±-Abi or 0.1nM DHT. Gene expression profiles are detected to determine the effect of 5a-Abi on prostate cancer cell line. LAPC4 cells were starved for 2 days with phenol red-free and serum free-medium and stimulated with 1ÂµM 5Î±-Abi or 0.1nM DHT for 48h. Gene expression profiles are detected to determine the effect of 5a-Abi on prostate cancer cell line.

2016-03-01 | E-GEOD-75387 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation

Project description:Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).The expression of HSD11B2 for the 3 experiments concerned was monitored in parallel by real time PCR which confirming significant downregulation by insulin.

2014-06-01 | GSE51677 | GEO

Qipengyuania sp. ABI-127-1

Project description:Qipengyuania sp. ABI-127-1 Genome sequencing and assembly

| PRJNA823897 | ENA

Argonaute2 mediates compensatory expansion of the pancreatic M-CM-^_-cell

Project description:Pancreatic M-CM-^_-cells adapt to compensate for the increased metabolic demand during insulin resistance. While the microRNA pathway has an essential role in the expansion of M-CM-^_-cell mass, the extent of its contribution is unclear. Here we show that miR-184 is silenced in the pancreatic islets of several insulin-resistant mouse models and in the islets of type-2 diabetic human subjects. Reduction of miR-184 promotes the expression of its target Argonaute2 (Ago2), a component of the microRNA-induced silencing complex. While over-expression of Ago2 increased M-CM-^_-cell proliferation, conditional deletion decreased M-CM-^_-cell number. Moreover, restored expression of miR-184 in leptin-deficient ob/ob mice decreased Ago2 and prevented compensatory M-CM-^_-cell expansion. Loss of Ago2 expression during insulin resistance blocked M-CM-^_-cell growth and relieved the regulation of miR-375-targeted genes including the growth suppressor Cadm1. This study identifies the regulation of Ago2 by miR-184 as an essential component of the compensatory response to promote proliferation during insulin resistance. MIN6 cells were transfected with Doxycyline responsive plasmids including the tetO-184 construct in biological triplicates for every time point. The conditions included untransfected control (CTR, induced), Transfected Control (TC, uninduced) along the time points of miR-184 overexpression in 16, 24, 48, and 72 hours of doxycycline treatment.

2014-02-06 | E-GEOD-46623 | biostudies-arrayexpress

The effect of human gingival fibroblast-conditioned medium (HGF-CM) on pulp regeneration

Project description:HGF-CM increases the proliferation and migration ability of DPSC (Dental pulp stem cells) in a dosage dependent manner, and facilitates the mineralization of DPSC by upregulating odotogenic genes. Although lack of in vitro evidence, DPSC and hGF-CM could be a promising combination for pulp regeneration in the future.

2022-10-29 | GSE201524 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data