Interplay Between Dox-Induced ICD and STING Pathway: Insights from Gene Expression in dendritic cells
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PROVIDER: PRJNA1006734 | ENA |
REPOSITORIES: ENA
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Project description:In this RNA sequencing study, the primary objectives were to explore the intricate interplay between the STING pathway and ICD-associated DAMPs in the context of treatment with cGAMP nanoparticle and Dox. The research delved deep into the transcriptomic alterations in BMDCs when exposed to cGAMPnps and conditioned medium from Dox-treated 4T1 breast cancer cells. By analyzing these shifts in gene expression and conducting a gene ontology analysis, the study sought to understand the inflammatory pathways and other gene clusters influenced by these treatments. Ultimately, this insight aims to inform the development of STING-mediated immunotherapies by identifying potential DAMP targets.
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Project description:BMDCs from WT, Fcgr2b-/- and Stinggt/gt were cultured, then stimulated with DMXAA for 3 and 6 hr. Cells were collected and lysed with 1 % IGEPAL CA-630, 0.5% TritonX-100, 150 mM NaCl, 50 mM Tris pH 7.4, 5% glycerol, 100 mM beta-Glycerophosphate, 2 mM Na3VO4 and 1X proteases inhibitor cocktail (Roche). First, antibody were mixed with the magnetic beads by adding 10 µg of STING antibody with 400 ug of SureBeads™ Protein A magnetic beads (Biorad, California, USA) and incubated for 1 hour at room temperature. Then, Protein lysates were added and incubated with antibody-conjugated beads for overnight at 4oC. After incubation, the beads were washed 3 times with wash buffer (150 mM NaCl and 50 mM Tris-HCL pH 7.4). Samples were eluted by adding 5X laemmli buffer and, boiled 950C for 10 minutes. The eluted protein samples were separated by 10 % SDS-PAGE gel. The STING interacting proteins from co-IP were analyzed by in-gel digestion followed by LC-MS/MS analysis.
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