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Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.

ABSTRACT: Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.

PROVIDER: PRJNA1007705 | ENA |

REPOSITORIES: ENA

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Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.

Project description:We established a dual reporter (SFTPC:GFP AGER:mCherry-HiBiT) human iPSC line and the "On-Gel" spheroid culture system suitable for finding the essential signals for alveolar type 2 (AT2) to type 1 (AT1) cell conversion comprehensively. After compound screening, we found that LATS-IN-1 and BAY1125976 synergistically promoted AT1 marker gene expression.

2024-02-29 | GSE241336 | GEO

Multicellular spheroids treated with the compound VLX600

Project description:Microarray based mRNA profiling was used to charactarize the response to the compound VLX600 in cells grown as spheroids. Cells used was colon cancer cells HCT116 and HCT116HIF1a knock-out. We identify the small molecule VLX600 as a drug that is preferentially active against quiescent cells in colon cancer 3-D microtissues. The anticancer activity is associated with reduced mitochondrial respiration, leading to a bioenergetic catastrophe and tumor cell death. VLX600 shows enhanced cytotoxic activity under conditions of nutrient starvation. Microarray based mRNA profiling was used to charactarize the response to the compound VLX600 in cells grown as spheroids. Cells used was colon cancer cells HCT116 and HCT116HIF1a knock-out cells.

2014-02-17 | E-GEOD-53777 | biostudies-arrayexpress

Multicellular spheroids treated with the compound VLX600

2014-02-17 | GSE53777 | GEO

Comparison of iPSC-derived alveolar epithelial cell culture methods (B2-3 SFTPC-GFP reporter iPSCs) and compound treatment in the alveolar epithelial spheroids (B2-3-dual-42-7 SFTPC-GFP AGER-mCherryHiBiT reporter iPSCs)

Project description:Comparison of iPSC-derived alveolar epithelial cell culture methods (B2-3 SFTPC-GFP reporter iPSCs) and compound treatment in the alveolar epithelial spheroids (B2-3-dual-42-7 SFTPC-GFP AGER-mCherryHiBiT reporter iPSCs)

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Project description:This SuperSeries is composed of the SubSeries listed below.

2024-02-29 | GSE241337 | GEO

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Gene epression profile of s-spheroids and t-spheroids differentiated from iPS cells

Project description:For definitive endoderm differentiation, iPS cells (day0) were treated with 100 ng ml-1 activin A and 3μM CHIR99021 for 1 day and 100 ng ml-1 activin A for the following two days. Definitive endoderm was subsequently treated with 3uM CHIR99021 and 500ng ml-1 FGF4 for mid/hindgut differentiation. Mid/hindgut floating spheroids (fl; t-Spheroids) were collected from culture medium from day6 to day 8. On day6, mid/hindgut cells were dissociated into single cells and seeded onto EZSPHERE plate to generate suspension spheroids(EZ; s-Spheroids). RNA of fl spheroids and EZ spheroids were extracted using QIAGEN RNeasy kit.

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GoldCLIP: Gel-omitted Ligation-dependent CLIP

Project description:GoldCLIP: Gel-omitted Ligation-dependent CLIP

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Genomic characterization of ovarian cancer spheroids

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2014-01-03 | GSE53759 | GEO

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