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Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.

ABSTRACT: Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.

PROVIDER: PRJNA1007705 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25715381_1.fastq.gz	Fastqsanger.gz
	SRR25715381_2.fastq.gz	Fastqsanger.gz
	SRR25715382_1.fastq.gz	Fastqsanger.gz
	SRR25715382_2.fastq.gz	Fastqsanger.gz
	SRR25715383_1.fastq.gz	Fastqsanger.gz

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Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.

Project description:We established a dual reporter (SFTPC:GFP AGER:mCherry-HiBiT) human iPSC line and the "On-Gel" spheroid culture system suitable for finding the essential signals for alveolar type 2 (AT2) to type 1 (AT1) cell conversion comprehensively. After compound screening, we found that LATS-IN-1 and BAY1125976 synergistically promoted AT1 marker gene expression.

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Multicellular spheroids treated with the compound VLX600

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Comparison of iPSC-derived alveolar epithelial cell culture methods (B2-3 SFTPC-GFP reporter iPSCs) and compound treatment in the alveolar epithelial spheroids (B2-3-dual-42-7 SFTPC-GFP AGER-mCherryHiBiT reporter iPSCs)

Project description:Comparison of iPSC-derived alveolar epithelial cell culture methods (B2-3 SFTPC-GFP reporter iPSCs) and compound treatment in the alveolar epithelial spheroids (B2-3-dual-42-7 SFTPC-GFP AGER-mCherryHiBiT reporter iPSCs)

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Project description:This SuperSeries is composed of the SubSeries listed below.

2024-02-29 | GSE241337 | GEO

Denunded HUVEC/HUASMC spheroids vs. HUASMC spheroids

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Gene epression profile of s-spheroids and t-spheroids differentiated from iPS cells

Project description:For definitive endoderm differentiation, iPS cells (day0) were treated with 100 ng ml-1 activin A and 3μM CHIR99021 for 1 day and 100 ng ml-1 activin A for the following two days. Definitive endoderm was subsequently treated with 3uM CHIR99021 and 500ng ml-1 FGF4 for mid/hindgut differentiation. Mid/hindgut floating spheroids (fl; t-Spheroids) were collected from culture medium from day6 to day 8. On day6, mid/hindgut cells were dissociated into single cells and seeded onto EZSPHERE plate to generate suspension spheroids(EZ; s-Spheroids). RNA of fl spheroids and EZ spheroids were extracted using QIAGEN RNeasy kit.

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