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ABSTRACT: Transcriptome of sugarcane SP80-3280 aerenchyma roots

PROVIDER: PRJNA1008343 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25737009.fastq.gz	Fastqsanger.gz
	SRR25737010.fastq.gz	Fastqsanger.gz
	SRR25737011.fastq.gz	Fastqsanger.gz

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Sugarcane transcriptome: Lxx inoculated (LxxI) vs. mocked inoculated (MI) plants

Project description:Transcriptional profile of sugarcane plants variety SP80-3280 inoculated with the pathogen Leifsonia xyli subsp. xyli (Lxx) compared with mocked inoculated ones 30 and 60 days after inoculation (DAI). Goal was to determine the effects of the increase in Lxx title on global sugarcane gene expression.

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Project description:Transcriptomic response of Sugarcane SP80-3280 at drought condition

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Sugarcane transcriptome - Phosphate starvation

Project description:Rooted sugarcane plantlets, originated from in vitro meristem culture (genotype SP80-3280, CTC, Brazil), were greenhouse acclimatized by initial cultivation on 1/20th strength Hoagland and Arnon (1950) nutrient solution. Nutrient solutions were aired from an oil-less compressor and replaced every 7 days, increasing nutrient concentration to ¼ strength in 3 weeks. Plants were then individually transferred to 2.8 L pots filled with fresh ¼ strength nutrient solution. After one week, half of the plants were transferred to fresh solution containing 250 µM Pi, while the other half was transferred to nutrient solution deprived of phosphate (Pi), with H2PO4 being replaced by H2SO4 (Muchhal et al., 1996). Roots from six plants from each treatment (0 and 250 µM Pi) were harvested 6, 12, 24 and 48 h after exposure to phosphate starvation and immediately frozen in liquid nitrogen. For each time point and each treatment, root samples were aggregated in three pools of two samples each. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

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