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Single-cell gene expression profiling of SOXC-mutant bone and bone marrow cells

ABSTRACT: Single-cell gene expression profiling of SOXC-mutant bone and bone marrow cells

PROVIDER: PRJNA1009025 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25756462.fastq.gz	Fastqsanger.gz
	SRR25756463.fastq.gz	Fastqsanger.gz
	SRR25756464.fastq.gz	Fastqsanger.gz
	SRR25756465.fastq.gz	Fastqsanger.gz
	SRR25756466.fastq.gz	Fastqsanger.gz

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Single-cell gene expression profiling of SOXC-mutant bone and bone marrow cells

Project description:SOXC genes (i.e., SOX4, SOX11 and SOX12) encode for transcription factors controlling survival, proliferation, commitment and differentiation of several cell types from development onwards. Although human diseases indicate crucial roles for SOXC in bone development and maintenance, SOXC activities in osteoblastic cells remain elusive. Here, we used a mouse model to inactivate SOXC genes in the osteoblast lineage cells using an OsxCre transgene (Sox4fl/fl Sox11fl/fl Sox12fl/fl OsxCre mice referred to as SOXCOsxCre mice) and we used single-cell RNA-sequencing assays to characterize gene expression changes in bone and bone marrow cells in adulthood.

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Project description:SOXC genes (i.e., Sox4, Sox11 and Sox12) are important members of the SOX transcription factor family. They are involved in several developmental processes, including skeleton formation. However, their role in intramembranous ossification and, particularly, in skull development has not beed investigated yet. Here, we employed single-cell RNA-seq technology to better understand the roles of the SOXC genes in craniogenesis and how their inactivation could affect this process. We identified the expression of the SOXC genes in several cranial populations, including osteodermal and meningeal progenitors. Removal of the SOXC genes in the cranial mesenchyme with a Prx1Cre transgene showed changes in the proportions of progenitor clusters and deriving populations (dermal, hypodermal, periosteal, suture and bone cells). Mutant progenitors showed the downregulation of genes involved in cytoskeleton organization, cell adhesion and migration, cell proliferation and steorl biosynthesis. As a result, SOXC-mutant fetuses have underdeveloped bones, sutures, dermis and hypodermis, particularly in the apical regions.

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