Project description:af09_lignin - stem developement - Transcriptome analysis of lignin mutants and UV stress effect on secondary wall synthesis - transcription frofiling of stem developement in Arabidopsis thaliana. time course with 3 points: 2cm, 10 cm and 25cm (maturation) Keywords: time course
Project description:af09_lignin - dml6 - Transcriptome analysis of lignin mutants and UV stress effect on secondary wall synthesis - DML6 vs S DML6+UV vs S+UV S vs S+UV DML6 vs DML6+UV Keywords: normal vs disease comparison,treated vs untreated comparison
Project description:ra07-01_prx34 - primary floral stem 40cm - The At3g49120 gene codes for the peroxidase 34 (PRX 34), an enzyme potentially involved in the polymérisation of monolignols, the lignin precursors. This enzyme is expressed in primary floral stem and its absence has an impact on the lignin quantity and biomass (at the young stage). Here, its question to characterize several mutants. - Each mutant compare to wild type, samples only primary floral stem of 40cm Keywords: normal vs transgenic comparaison
Project description:ra07-01_prx34 - primary floral stem 20cm - The At3g49120 gene codes for the peroxidase 34 (PRX 34), an enzyme potentially involved in the polymérisation of monolignols, the lignin precursors. This enzyme is expressed in primary floral stem and its absence has an impact on the lignin quantity and biomass (at the young stage). Here, its question to characterize several mutants. - Each mutant compare to wild type, samples only primary floral stem of 20cm Keywords: normal vs transgenic comparaison
Project description:UV-B radiation regulates numerous morphogenic, biochemical and physiological responses in plants, and can stimulate some responses typically associated with other abiotic and biotic stimuli, including invertebrate herbivory. Removal of UV-B from the growing environment of various plant species has been found to increase their susceptibility to consumption by invertebrate pests, however, to date, little research has been conducted to investigate the effects of UV-B on crop susceptibility to field pests. Here, we report findings from a multi-omic and genetic-based study investigating the mechanisms of UV-B-stimulated resistance of the crop, Brassica napus (oilseed rape), to herbivory from an economically important lepidopteran specialist of the Brassicaceae, Plutella xylostella (diamondback moth). The UV-B photoreceptor, UV RESISTANCE LOCUS 8 (UVR8), was not found to mediate resistance to this pest. RNA-Seq and untargeted metabolomics identified components of the sinapate/lignin biosynthetic pathway that were similarly regulated by UV-B and herbivory. Arabidopsis mutants in genes encoding two enzymes in the sinapate/lignin biosynthetic pathway, CAFFEATE O-METHYLTRANSFERASE 1 (COMT1) and ELICITOR-ACTIVATED GENE 3-2 (ELI3-2), retained UV-B-mediated resistance to P. xylostella herbivory. However, overexpression of B. napus COMT1 in Arabidopsis further reduced plant susceptibility to P. xylostella herbivory in a UV-B-dependent manner. These findings demonstrate that overexpression of a component of the sinapate/lignin biosynthetic pathway in a member of the Brassicaceae can enhance UV-B-stimulated resistance to herbivory from P. xylostella.
Project description:ra07-01_prx34 - primary floral stem 40cm - The At3g49120 gene codes for the peroxidase 34 (PRX 34), an enzyme potentially involved in the polymérisation of monolignols, the lignin precursors. This enzyme is expressed in primary floral stem and its absence has an impact on the lignin quantity and biomass (at the young stage). Here, its question to characterize several mutants. - Each mutant compare to wild type, samples only primary floral stem of 40cm Keywords: normal vs transgenic comparaison 2 dye-swap - CATMA arrays
Project description:ra07-01_prx34 - primary floral stem 20cm - The At3g49120 gene codes for the peroxidase 34 (PRX 34), an enzyme potentially involved in the polymérisation of monolignols, the lignin precursors. This enzyme is expressed in primary floral stem and its absence has an impact on the lignin quantity and biomass (at the young stage). Here, its question to characterize several mutants. - Each mutant compare to wild type, samples only primary floral stem of 20cm Keywords: normal vs transgenic comparaison 4 dye-swap - CATMA arrays
Project description:ra10-01_laccases; laccase mutations. We demonstrated that laccases are involved in lignin polymerisation. Mutants have already been tested on microarrays and there is few differences compared to wild-type. The laccase mutation seems surgical. We possess a new double mutant, called snips, with a semi-dwarf phenotype, and we want to determine its profile.
