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RNA-seq analysis of knockdown of ANXA2 in uterine carcinosarcoma cell line FU-MMT-1 cells.

ABSTRACT: RNA-seq analysis of knockdown of ANXA2 in uterine carcinosarcoma cell line FU-MMT-1 cells.

PROVIDER: PRJNA1010199 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25779244_1.fastq.gz	Fastqsanger.gz
	SRR25779244_2.fastq.gz	Fastqsanger.gz
	SRR25779245_1.fastq.gz	Fastqsanger.gz
	SRR25779245_2.fastq.gz	Fastqsanger.gz
	SRR25779246_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of knockdown of ANXA2 in uterine carcinosarcoma cell line FU-MMT-1 cells.

Project description:Uterine carcinosarcomas (UCS) are rare highly aggressive gynecological malignancy. ANXA2 is a calcium-dependent phospholipid binding protein that is over-expressed in various cancers, and plays a pivotal role in tumor migration, metastasis. However, the exact function and mechanism of ANXA2 in UCS have not been elucidated. Herein, we aim to elucidate the role of ANXA2 in UCS and the molecular mechanism involved in it.

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CD133+ cells contain cancer stem cell-like properties in Malignant Mixed Mullerian Tumor (Carcinosarcoma) of the Uterus

Project description:Cancer stem cells (CSCs) that display tumor-initiating properties have recently been identified. We herein identify and characterize CSCs in human uterine carcinosarcoma, a highly aggressive and therapy-resistant gynecologic malignancy, which is considered to be of mesodermal origin. FU-MMT-1, a cell-line, which was established by us (Emoto M, Cancer 1992) from a patient with uterine carcinosarcoma, was evaluated. FU-MMT-1 contained a high population of CD133, CD44, CD90, and CD29 positive cells. Using the magnetic bead cell separation method, we isolated CD133+ cells, which predominantly form spheres in culture. These CD133+ cells form transplantable tumors in vivo. A qRT-PCR analysis of the genes implicated in stem cell maintenance revealed that CD133+ cells express significantly higher levels of OCT4, NANOG, and BMI-1 than CD133M-oM-<M- cells. Moreover, CD133+ cells showed a high expression of PAX2 and WNT4, which are the essential genes in Mullerian duct formation. The tumor derived from CD133+ cells replicated vimentin, ERM-NM-1, ERM-NM-2, and PR expressionsM-cM-^@M-^@of the parent tumor. These findings suggest that CD133+ FU-MMT-1 cells have the characteristics of CSCs and Mullerian mesenchymal progenitors. CD133+ and CD133- population of FU-MMT-1 cells were analyzed by microarray.

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CD133+ cells contain cancer stem cell-like properties in Malignant Mixed Mullerian Tumor (Carcinosarcoma) of the Uterus

Project description:Cancer stem cells (CSCs) that display tumor-initiating properties have recently been identified. We herein identify and characterize CSCs in human uterine carcinosarcoma, a highly aggressive and therapy-resistant gynecologic malignancy, which is considered to be of mesodermal origin. FU-MMT-1, a cell-line, which was established by us (Emoto M, Cancer 1992) from a patient with uterine carcinosarcoma, was evaluated. FU-MMT-1 contained a high population of CD133, CD44, CD90, and CD29 positive cells. Using the magnetic bead cell separation method, we isolated CD133+ cells, which predominantly form spheres in culture. These CD133+ cells form transplantable tumors in vivo. A qRT-PCR analysis of the genes implicated in stem cell maintenance revealed that CD133+ cells express significantly higher levels of OCT4, NANOG, and BMI-1 than CD133－ cells. Moreover, CD133+ cells showed a high expression of PAX2 and WNT4, which are the essential genes in Mullerian duct formation. The tumor derived from CD133+ cells replicated vimentin, ERα, ERβ, and PR expressions　of the parent tumor. These findings suggest that CD133+ FU-MMT-1 cells have the characteristics of CSCs and Mullerian mesenchymal progenitors.

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