Project description:Transcriptional profiling of human prostate and breast epithelial cells comparing control siRNA-treated with EZH2-siRNA treated Keywords: Genetic modification
Project description:To determine the altered mRNA expression signatures upon overexpressing EZH2 regulated microRNAs in cancer. To identify EZH2 regulated microRNAs in cancer. Two-condition experiment: Each EZH2 microRNA was overexpressed and compared to a control RNA in BT549 breast cancer cells. In DU145 prostate cancer cell EZH2 was knocked down and comapred to EZH2 scrambled siRNA. Other cell lines were monitored for miRNA expression as they are known to have high endogenous levels of EZH2.
Project description:Transcriptional profiling of human prostate and breast epithelial cells with a time course analysis of EZH2 overexpression of cells at 3, 6, 12, 24, 48, and 72 hrs respectively. Keywords: Genetic modification
Project description:This study aimed to elucidate the role of ELF3, an epithelia-specific member of the ETS family, in normal prostate development and prostate cancer. Four comparisons (untreated, mock-transfected, scrambled siRNA, and targeted siRNA) were performed in two prostate epithelial cell lines BPH-1 (benign) and PC3 (malignant). Global gene expression arrays were performed for each combination.
