Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood.

ABSTRACT: The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood.

PROVIDER: PRJNA1013081 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood [scRNA-seq]

2023-10-19 | GSE241266 | GEO

The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood [Bulk RNA-seq]

Project description:Peripheral monocytes in humans are conventionally divided into classical (CL, CD14++CD16−), intermediate (INT, CD14++CD16+) and non-classical (NC, CD14dim/−CD16++) cells, based on their expression levels of CD14 and CD16. A major fraction of the NC-monocytes has been shown to express the 6-sulfo LacNAc (slan) antigen, but whether these slan+/NC-monocytes represent the prototypical non-classical monocytes or whether they are simply a sub-fraction with identical features as the remainder of NC monocytes is still unclear. Therefore, we analysed transcriptome, proteome, cell surface markers and production of discrete cytokines by peripheral slan+/NC- and slan−/NC-monocytes, in comparison to total NC-, CL- and INT- monocytes. By bulk RNA-seq and proteomic analysis, we found that slan+/NC-monocytes express higher levels of genes and proteins specific of NC-monocytes than slan−/NC-monocytes do. Unsupervised clustering of scRNA-seq data generated one cluster of NC- and one of INT-monocytes, where all slan+/NC-monocytes were allocated to the NC-monocyte cluster, while slan−/NC-monocytes were found, in part (13.38 %), within the INT-monocyte cluster. In addition, total NC- and slan−/NC-monocytes, but not slan+/NC-monocytes, were found by both bulk RNA-seq and scRNA-seq to contain a small percentage of natural killer cells. Altogether, in addition to comparatively characterize total NC-, slan−/NC- and slan+/NC-monocyte transcriptomes and proteomes, our data prove that slan+/NC-, but not slan−/NC-, monocytes are more representative of prototypical NC-monocytes.

2023-10-19 | GSE242301 | GEO

The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood [scRNA-seq]

Project description:The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood [scRNA-seq]

| PRJNA1007605 | ENA

The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood [Bulk RNA-seq]

Project description:The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood [Bulk RNA-seq]

| PRJNA1013061 | ENA

Transcriptomic analysis of SLAN+ vs SLAN- non-classical monocytes

Project description:Monocytes can be classified into four different subsets: classic, intermediate and SLAN- and SLAN+ non-classical monocytes. SLAN+ monocytes are significantly reduced in MM patients compare to the frequency found in healthy adults (HA). Thus, we sought to analyze the transcriptome of SLAN- and SLAN+ cells to unveil the differences among both populations We used microarrays to detail the global programme of gene expression underlying the differences among both cell subsets We developed a 12-color combination of mAbs to evaluate the contribution of each individual marker for identifying and subsetting BM TAMs in patients with MM (N = 5): CD36-UV405, CD16-UV525, CD163-BV450, CD45-BV525, CD206-BV610, CD86-BV660, CD62L-BV763, cyCD68-FITC, SLAN-PE, HLADR-PerCPCy5.5, CD300e-APC, CD14-APCH7. This panel allowed us to identify the aforementioned four monocyte subsets. Intermediate and non-classical monocytes from healthy donors were FACS sorted based on the 7-color mAb combination - HLADR-PacB, CD45-OC515, CD36-FITC, SLAN-PE, CD16-PECy7, CD300e-APC, CD14APCH7 -

2023-11-05 | GSE122171 | GEO

The slan antigen identifies the prototypical non-classical CD16<sup>+</sup>-monocytes in human blood.

Project description:IntroductionPeripheral monocytes in humans are conventionally divided into classical (CL, CD14++CD16-), intermediate (INT, CD14++CD16+) and non-classical (NC, CD14dim/-CD16++) cells, based on their expression levels of CD14 and CD16. A major fraction of the NC-monocytes has been shown to express the 6-sulfo LacNAc (slan) antigen, but whether these slan+/NC-monocytes represent the prototypical non-classical monocytes or whether they are simply a sub-fraction with identical features as the remainder of NC monocytes is still unclear.MethodsWe analyzed transcriptome (by bulk and single cell RNA-seq), proteome, cell surface markers and production of discrete cytokines by peripheral slan+/NC- and slan-/NC-monocytes, in comparison to total NC-, CL- and INT- monocytes.ResultsBy bulk RNA-seq and proteomic analysis, we found that slan+/NC-monocytes express higher levels of genes and proteins specific of NC-monocytes than slan-/NC-monocytes do. Unsupervised clustering of scRNA-seq data generated one cluster of NC- and one of INT-monocytes, where all slan+/NC-monocytes were allocated to the NC-monocyte cluster, while slan-/NC-monocytes were found, in part (13.4%), within the INT-monocyte cluster. In addition, total NC- and slan-/NC-monocytes, but not slan+/NC-monocytes, were found by both bulk RNA-seq and scRNA-seq to contain a small percentage of natural killer cells.ConclusionIn addition to comparatively characterize total NC-, slan-/NC- and slan+/NC-monocyte transcriptomes and proteomes, our data prove that slan+/NC-, but not slan-/NC-, monocytes are more representative of prototypical NC-monocytes.

| S-EPMC10641684 | biostudies-literature

Transcriptomic analysis of SLAN+ vs SLAN- non-classical monocytes

Project description:Transcriptomic analysis of SLAN+ vs SLAN- non-classical monocytes

| PRJNA503867 | ENA

The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-10-19 | GSE242314 | GEO

Deciphering the fate of slan+-monocytes in tonsils by gene expression profiling

Project description:slan+-monocytes represent a subset of the “non-classical” CD14dim/-CD16++ monocytes, which are characterized by the expression of the so-called “slan” antigen, recognized by specific monoclonal antibodies. slan+-cells can be identified in peripheral tissues including tonsils. However, their origin from slan+-monocytes as well as their terminal differentiation state (toward macrophages/dendritic cells) were only partially resolved. In this study, we performed transcriptomic studies of tonsil slan+-cells,CD1c+DCs/DC2 and CD11b+ CD14+macrophages, as well as subsets of peripheral blood monocytes and we report that tonsil slan+-cells mainly represent macrophage-like cells with features distinct from those of tonsil CD11b+CD14+macrophages or CD1c+DCs/DC2. Moreover we provide a number of molecular evidence indicating that tonsil slan+-cells derive from peripheral slan+-monocytes.

2021-11-19 | GSE136107 | GEO

Gene expression profiles of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (CD14+16++ and CD14++CD16+), and CD1c+ CD19- dendritic cells [human data]

Project description:In this study gene expression of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (consisting of non-classical CD14+16++ and intermediate CD14++CD16+ monocytes) and CD1c+ CD19- dendritic cells from healthy subjects were investigated. Keywords: expression profiling by array

2011-12-17 | GSE34515 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data