Project description:This study looks at the gene expression of two chain-elongating bacteria, Pseudoramibacter alactolyticus and Megasphaera elsdenii, in monoculture and in coculture with anaerobic gut fungi (Neocallimastix lanati). P. alactolyticus in particular has appeared stable in synthetic cocultures with anaerobic fungi based on metabolic output, and this RNA-seq study was designed to further assess that stability. Additionally, chain-elongating bacteria can use fungal fermentation products to generate short- and medium-chain fatty acids, and this study was employed to see how genes involved in this process (reverse beta oxidation) were regulated in coculture compared to monoculture.
Project description:Metagenomics analysis reveals co-infection of fungi and bacteria isolated from different regions of brain tissue from elderly persons and patients with Alzheimer's disease.
Project description:Interventions: ntestinal polyp gruop and colorectal cancer gruop:Nil
Primary outcome(s): bacteria;fungi;archaea;virus
Study Design: Factorial
Project description:Interventions: healthy people, intestinal polyp group and intestinal cancer group.:Nil
Primary outcome(s): bacteria;fungi;phages
Study Design: Factorial
Project description:In order to get insights into the ability of ectomycorrhizal fungi to perceive their biotic environment as well as into the mechanisms of the interactions between ectomycorrhizal fungi and soil bacteria, we analysed the transcriptomic response of the ectomycorrhizal fungus L. bicolor and of two beneficial, and neutral soil bacteria during their interactions in vitro.