Project description:We found that the number of tumor-infiltrating myofibroblasts was positively correlated to tumor acidification status in hepatocellular carcinoma (HCC). Hepatic stellate cells (HSCs), the predominant precursors of liver myofibroblasts, were activated and transdifferentiated into myofibroblasts under acidic culture condition. To identify the molecular phenotype of LX-2 cells in acidic culture conditions, we further conducted a gene expression profile analysis. LX-2 cells cultured in pH 7.2 or pH 6.2 medium separately for six days was used in gene expression microarray analysis.

2013-07-29 | E-GEOD-49301 | biostudies-arrayexpress

Transcriptome of human LX-2 hepatic stellate cells treated with the OGT inhibitor OSMI-1

Project description:Transcriptome of human LX-2 hepatic stellate cells treated with the OGT inhibitor OSMI-1

| PRJNA1016368 | ENA

Transcriptome of activated mouse primary hepatic stellate cells treated with the OGT inhibitor OSMI-1

Project description:RNA-seq analysis of mouse primary myofibroblastic hepatic stellate cells (pMF-HSCs) treated or not with OSMI-1 OGT inhibitor (OGTi).

2024-05-22 | GSE243106 | GEO

Human hepatic stellate cell line LX-2 activated under acidic culture condition

2013-07-29 | GSE49301 | GEO

Expression data from the hepatic stellate cell line LX-2 after treatment with DMOG and from the liver sinusoidal endothelial cell line TRP3 after incubation with conditioned medium of DMOG-treated LX-2

Project description:Expression data from the hepatic stellate cell line LX-2 after treatment with the prolylhydroxylase inhibitor dimethyloxalylglycine (DMOG) and from the liver sinusoidal endothelial cell line TRP3 after incubation with conditioned medium of DMOG-treated LX-2 Prolyl-hydroxylase inhibitors such as dimethyloxalylglycine (DMOG) stabilize HIF-1α, thereby chemically inducing hypoxia, which also accelerates liver volume increase when given with portal rerouting. We used microarrays to clarify the cellular crosstalk of the different cell types in accelerated liver regeneration by examining the role of hepatic stellate cells (HSC) and liver sinusoidal endothelial cells (LSEC).

2020-03-23 | GSE131168 | GEO

MicroRNA expression profile of human hepatic stellate cells LX-2 co-cultured with human umbilical cord mesenchymal stem cells and control group

Project description:Hepatic stellate cells(HSCs) are the main effector cells of liver fibrosis. In order to study the effect of mesenchymal stem cells(MSCs) on microRNAs expression of HSCs, we co-cultured HSCs LX-2 activated by TGFβ1 with human umbilical cord MSCs(hUC-MSCs) for 48 hours, and compared the differentially expressed miRNA with LX-2 cultured alone by high-throughput sequencing. The results showed that two mature microRNAs expressed increased, and nine expressed decreased.

2020-05-24 | GSE151098 | GEO

Transcriptome of human LX-2 hepatic stellate cells transfected with siRNA directed against OGT

Project description:RNA-seq analysis of human LX-2 cells treated transfected or not with siOGT.

2024-05-22 | GSE243105 | GEO