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Cellular and transcriptional signatures unveiled by single cell RNA-Seq (scRNA-Seq) following ex vivo infection of murine splenocytes with Borrelia burgdorferi

ABSTRACT: Cellular and transcriptional signatures unveiled by single cell RNA-Seq (scRNA-Seq) following ex vivo infection of murine splenocytes with Borrelia burgdorferi

PROVIDER: PRJNA1016403 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26056400_1.fastq.gz	Fastqsanger.gz
	SRR26056400_2.fastq.gz	Fastqsanger.gz
	SRR26056401_1.fastq.gz	Fastqsanger.gz
	SRR26056401_2.fastq.gz	Fastqsanger.gz
	SRR26056402_1.fastq.gz	Fastqsanger.gz

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Cellular and transcriptional signatures unveiled by single cell RNA-Seq (scRNA-Seq) following ex vivo infection of murine splenocytes with Borrelia burgdorferi

Project description:Splenocytes comprised a wide-range of immune cells responsible for the protection against infectious diseases. We exposed splenocytes to GFP+ Borrelia, sorted GFP+ and GFP- splenocytes and performed single-cell RNA seq to analyze the DEGs between infected and bystander population in different immune cell populations

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microRNA changes in astrocytes following Borrelia burgdorferi infection

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Project description:RNA was isolated from late-log pahse wild-type Borrelia burgdorferi B31 and the bb0647 mutant grown in BSKH media at 37 degree, 5% CO2. cDNA was synthesized, labeled and hybridized to the 70mer oligonucleotide B. burgdorferi array. Slides were scanned using axon scanner and image were analyzed using GenePix 6.0. Dta were further analysed using the professional software Acuity 4.o, based on a ratio-based normalization. Samples were labeled by either Cy3 or Cy5. 5 hybs were performed, including dye-swaps.

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Project description:Differential gene expression analysis was performed to assess the affects of the deletion of sRNA SR0726 on the in vitro transcriptome of Borrelia burgdorferi in order to investigate a potential regulatory role for the sRNA

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Project description:Background: Lyme borrelia genotypes differ in their capacity to cause disseminated disease. Gene array analysis was employed to profile the host transcriptome induced by Borrelia burgdorferi strains with different capacities for causing disseminated disease in the blood of C3H/HeJ mice during early infection. Results: Borrelia burgdorferi B515, a clinical isolate that causes disseminated infection in mice, differentially regulated 236 transcripts (P<0.05 by ANOVA, with fold change of at least 2). The 216 significantly induced transcripts included IFN-responsive genes and genes involved in immunity and inflammation. In contrast, B. burgdorferi B331, a clinical isolate that causes transient skin infection but does not disseminate in C3H/HeJ mice, stimulated changes in only a few genes (1 induced, 4 repressed). Transcriptional regulation of type I IFN and IFN-related genes was measured by quantitative RT-PCR in mouse skin biopsies collected from the site of infection 24 hours after inoculation with B. burgdorferi. The mean values for transcript of Ifnb, Cxcl10, Gbp1, Ifit1, Ifit3, Irf7, Mx1, and Stat2, were found to be significantly increased in B. burgdorferi strain B515-infected mice relative to the control group. In contrast, transcription of these genes was not significantly changed in response to B. burgdorferi strain B331 or B31-4, a mutant that is unable to disseminate. Conclusions: These results establish a positive association between the disseminating capacity of B. burgdorferi and early type I IFN induction in a murine model of Lyme disease.

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