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ABSTRACT: Spatial Transcriptomics of chicken pectoralis major muscle

PROVIDER: PRJNA1017808 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26070077_1.fastq.gz	Fastqsanger.gz
	SRR26070077_2.fastq.gz	Fastqsanger.gz
	SRR26070078_1.fastq.gz	Fastqsanger.gz
	SRR26070078_2.fastq.gz	Fastqsanger.gz
	SRR26070079_1.fastq.gz	Fastqsanger.gz

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Genome-wide transcriptome analysis of chickens during dietary methionine restriction reveals compromised immune competency

Project description:The goal of this study was to compare next-generation sequencing (NGS)-derived liver and pectoralis major transcriptome profilings (RNA-seq) of chicken fed control diet to treatment birds fed methionine restriction diet. We sequenced mRNA from 10 Gallus gallus males fed either control (L-Methionine) or L-Methionine-deficient diet (5 control and 5 treatment) from 3-5 weeks of age. We sequenced both liver and the pectoralis major muscle.

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Divergent Gene Expression through PI3K/Akt Signaling Pathway Cause Different Models of Hypertrophy Growth in Chicken

Project description:RNA-Seq technology was used to investigate differences in the gene expression of pectoralis muscle tissue between two chicken breeds (Ross as commercial (rapidly growing) and Isfahani as Iranian local breed (slow-growing)).

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Gene expression profile in the pectoralis muscle of penguin before and after acclimatisation to marine life

Project description:In the present study, we were interested in gene expression changes in the pectoralis muscle of juvenile king penguins during the transition from terrestrial to marine life. Strictly terrestrial during their first year after hatching, king penguin chicks must then depart to sea to reach nutritional emancipation and pectoralis muscle is largely involved in penguin adaptation to the marine environment. To compare these transcriptomic profiles, we realized heterologous hybridization on Affymetrix GeneChip Chicken Genome Arrays, as the chicken is the closest model species for which microarrays are available. The development of a new algorithm, MaxRS, allow us to determine differentially expressed genes implicated in energetic metabolism or involved in cellular defense against reactive oxygen species and associated injuries.

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Gene expression profile in the pectoralis muscle of penguin before and after acclimatisation to marine life

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Transcriptome analysis of post-hatch breast muscle in legacy and modern broiler chickens reveals enrichment of several regulators of myogenic growth

Project description:We report the results of comparative transcriptional profiling in the pectoralis major muscle of a modern production broiler line (Ross 708) and a legacy broiler line inbred since the late 1940s (Illinois) focusing on metabolic differences and differences in myogenic growth regulators Differential gene expression analysis between Ross 708 and Illinois pectoralis major at two post-hatch time points (D6 and D21) that bracket critical inflection point in the allometric growth characteristics of the muscle

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Transcriptome profiling of the liver and pectoralis major muscle in slow-growing chickens under an organic raising system

Project description:The strategy to improve the competitiveness of smallholder farmers and to serve highly nutritious meat for consumers is the major goal of Korat chicken (KRC) production in Thailand. Organic KRC meat contains high n-3 polyunsaturated fatty acids (PUFA) and docosahexaenoic acid (DHA), which have a wide range of health-related benefits. However, limited scientific investigations have been conducted on the modulations of PUFA and DHA in KRC. In this study, we compared two rearing systems, conventional (CO) and organic raising (OR) systems, and their effects on global gene expression related to the accumulation of n-3 PUFA and DHA in the liver and pectoralis major muscle were investigated by using RNA-sequencing (RNA-seq). Total RNA was isolated from the liver and pectoralis major muscle of male KRC in each treatment group (n = 5). RNA-seq revealed a total of 561 and 361 differentially expressed genes between CO and OR groups identified in the liver and pectoralis major muscle, respectively. ELOVL fatty acid elongase 2 (ELOVL2) may activate n-3 PUFAs synthesis via the fatty acid elongation pathway. The KRC seems suitable to raise under the organic raising system, since it improved lipid metabolism and immune response in the liver. However, thermal environmental management and growth performance need to be considered. ELOVL2 can use as a gene marker for n-3 PUFAs synthesis in the liver. This study provides a genetic basis for future breeding strategies to improve the genetics of slow-growing chickens to fit with an organic raising system.

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