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ABSTRACT: Methanogen and Methanotroph sequencing in eastern China

PROVIDER: PRJNA1018681 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR26120700.fastq.gz	Fastqsanger.gz
	SRR26120700_1.fastq.gz	Fastqsanger.gz
	SRR26120700_2.fastq.gz	Fastqsanger.gz
	SRR26120701_1.fastq.gz	Fastqsanger.gz
	SRR26120701_2.fastq.gz	Fastqsanger.gz

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Project description:To obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in China, next-generation sequencing technology was performed on E-L 31, 35 and 38 stages of Cabernet Sauvignon grape berries from Changli (CL, eastern) and Gaotai (GT, western). The transcript abundance of epoxycarotenoid dioxygenase and xanthoxin dehydrogenase required for ABA biosynthesis was significantly higher in the GT berries at E-L 35 and 38 stages compared with the CL berries, which may explain the relatively short maturation period of berries in the western region. Some genes required for carbohydrate metabolism, such as hexose transporter, L-idonate dehydrogenase and phosphoenolpyruvate carboxylase, were significantly up-regulated in the CL berries in relative to the GT berries, which positively correlated with the sugar and organic acid accumulations. Pathway enrichment analysis of differentially expressed genes revealed that the CL berries had higher levels of phenylpropanoid biosynthesis at E-L 38 stage than the GT berries, which may relate to the quick fading of the GT wines because of weak co-pigmentation. cDNA libraries generated from three developmental stages (E-L 31, 35 and 38) of Cabernet Sauvignon grape berries from Changli (CL, eastern) and Gaotai (GT, western) in China were sequenced using Illumina HiSeq 2000.

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