Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

FoxP3 mRNA-seq in mouse CD4+T cells

ABSTRACT: FoxP3 mRNA-seq in mouse CD4+T cells

PROVIDER: PRJNA1018989 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26113722_1.fastq.gz	Fastqsanger.gz
	SRR26113722_2.fastq.gz	Fastqsanger.gz
	SRR26113723_1.fastq.gz	Fastqsanger.gz
	SRR26113723_2.fastq.gz	Fastqsanger.gz
	SRR26113724_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

FoxP3 mRNA-seq in mouse CD4+T cells

Project description:To investigate the impact of FoxP3 multimerization mutants on FoxP3's genome-wide gene expression profiles, we conducted FoxP3 RNA-seq experiments using mouse CD4+ T cells. The results indicated that all of these multimerization mutants had an effect on FoxP3 gene expression profiles, including some well-established Treg signatures.

2023-10-09 | GSE243602 | GEO

Comparative proteomic analysis of mouse CD4+Foxp3+ regulatory T cells and CD4+Foxp3- conventional T cells

Project description:In this study, we compared the proteomes of mouse CD4+Foxp3+ regulatory T cells (Treg) and CD4+Foxp3- conventional T cells (Tconv) in order to build a data set of proteins differentially regulated in these two cell populations. The data set contains mass spectrometry results from the analysis of 7 biological replicates of Treg/Tconv cell samples purified by flow cytometry, each experiment performed from a pool of 4-5 mice. Global proteomic analysis of each sample was performed by single-run nanoLC-MS/MS, using chromatographic separation of peptides on 50cm C18 reverse-phase columns, with either a 480min gradient on LTQ-Velos orbitrap mass spectrometer (replicates 1 and 2) or a 300min gradient on Q-Exactive orbitrap mass spectrometer (replicates 3-7). Several MS injection replicates were performed for some experiments, leading to 27 raw files composing the data set. The detailed description of each analysis (file name, sample type, biological replicate number, MS technical replicate number, MS instrument used, sample name in MaxQuant ouput) is given in the table “Files list.txt”.

2017-04-10 | PXD004436 | Pride

FoxP3 ChIP-seq in mouse CD4+T cells

Project description:FoxP3 ChIP-seq in mouse CD4+T cells

| PRJNA1018988 | ENA

FoxP3 ChIP-seq in mouse CD4+T cells

Project description:To investigate the impact of FoxP3 multimerization mutants on FoxP3's genome-wide DNA binding, with a particular focus on TnG-repeats binding, we conducted FoxP3 ChIP-seq experiments using mouse CD4+ T cells. The results revealed that all of these multimerization mutants have the potential to influence FoxP3 DNA binding, including its interaction with TnG-repeats.

2023-10-09 | GSE243599 | GEO

Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from secondary lymphoid organs

Project description:Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from secondary lymphoid organs

2018-02-14 | GSE110547 | GEO

Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from spleen and colon [MouseTreg_ColonSpleenSPFGF]

Project description:Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from spleen and colon

2018-02-14 | GSE110568 | GEO

Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from spleen and visceral adipose tissue

Project description:Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from spleen and visceral adipose tissue

2018-02-16 | GSE110688 | GEO

Single cell transcriptomic analysis of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from secondary lymphoid organs, colon, muscle, visceral adipose tissue [CelSeq]

Project description:Single cell transcriptomic analysis of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from secondary lymphoid organs, colon, muscle, visceral adipose tissue

2018-01-27 | GSE109726 | GEO

AIM2 negatively regulate FOXP3 expression in mouse CD4+ T cells

Project description:AIM2 negatively regulate FOXP3 expression in mouse CD4+ T cells

| PRJNA637234 | ENA

Single-cell RNAseq (10x Genomics) analysis of mouse splenic CD4+ T cells in WT and ∆Foxp3 mice and in WT/∆Foxp3 bone marrow chimeras

Project description:Single-cell RNAseq (10x Genomics) analysis of mouse splenic CD4+ T cells in WT and ∆Foxp3 mice and in WT/∆Foxp3 bone marrow chimeras. Mouse CD4+T cells in 21-day-old male WT and ∆Foxp3 mice were isolated from spleen by flow cytometry as DAPI–TCRβ+CD4+ cells for 10x Genomics Single Cell 3′ Reagent Kit (V2 chemistry, one sample per channel). For bone marrow chimera experiment: 7 week-old CD45.2-recipient mice were irradiated with 1000 Rad, reconstituted with 4 million CD3-depleted bone marrow cells: 50% CD45.1 x Foxp3-IRES-GFP (WT, 21d-old male) and 50% Foxp3DeltaEGFPiCre/RFP x ROSA-YFP x CD45.1/2 (scurfy, 21d-old male). 10 weeks later, spleen were harvested and tagged using a different Hashtags fo each mouse. ∆Foxp3 CD4+ T cells were sorted as DAPI–TCRb+CD4+CD45.1+CD45.2+. WT CD4+ T cells were sorted as DAPI–TCRb+CD4+CD45.1+CD45.2–. Control WT DAPI–TCRb+CD4+GFP+ Treg cells and GFP- Tconvs cells were also tagged and sorted. Samples with different hastags were pooled before single cell encapsulation using 10x Genomics Single Cell 3′ Reagent Kit (V3 chemistry).

2021-02-26 | GSE167575 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data