ABSTRACT: Gene expression profile at single cell level of leukocytes from the hearts of 5-day-old mice that underwent apical resection and Triiodothyronine treatment
Project description:Apical resection in 1-day-old mice results in complete regenerated heart and normal cardiac function, which is characterized by significant cardiomyocyte proliferation with minimal fibrosis. Local damage on hearts of 1-day-old mice can amplify cardiomyocyte proliferation located in the whole ventricle rather than limited damage area. This response provides a reasonable screening model to identify novel genes involved in endogenous cardiac regeneration
Project description:We report the transcriptional changes occuring in fibroblasts obtained from mouse hearts subjected to apical resection surgery or sham surgery, compared to unmanipulated controls, at indicated timepoints.
Project description:Next Generation RNAseq of neonatal hearts one day post surgery comparing sham, apical resection or apical resecection plus vagotomy treatments
Project description:We use spatial transcriptomics to establish regional transcriptional profile of neonatal heart tissue obtained at indicated timpoints after apical resection surgery.
Project description:Defining conserved molecular pathways in animal models of successful cardiac regeneration could yield insight into why adult mammals have inadequate cardiac regeneration after injury. Here we describe a cross-species transcriptomic screen to identify evolutionarily conserved pathways in the early events of cardiac regeneration in three species that can regenerate myocardium after a major injury. In this study, we performed RNA-seq on regenerating hearts from three model organisms - axolotl, zebrafish and mouse. Apical resection was performed to amputate ~10 - 20% of the left ventricle in all three model organisms. Following resection, hearts were harvested at 12, 24 and 48 hours post-resection and subjected to RNA-seq. RNA-seq on sham controls (no ventricular amputation) was used as interanal control. This approach revealed upregulation of inflammatory genes in all three organisms during regeneration. Furthermore, upregulation of Complement 5a receptor1 (C5aR1) expression in the regenerating hearts of zebrafish, axolotls and mice was observed.
Project description:Pneumonectomy, resection of left lung lobe, induces regenerative responses that consist of numerous genetic changes. To identify genetic changes, we compare the isolated vascular cells from young and old mice that underwent pneumonectomy.
Project description:Interventions: Group 1: On the day of the reconnaissance, the blood sampling with CRP, leukocytes, PCT, lactate and calprotectin. The next blood samples (CRP, leukocytes, PCT, lactate and Calprotectin) are performed on the 1st, 3rd and 5th postoperative day.
Primary outcome(s): How does the calprotectin level in the serum behave pre- and post-operatively after a colon / rectum resection?
Study Design: Allocation: ; Masking: ; Control: ; Assignment: ; Study design purpose: prevention
Project description:Background: In this study, we have extended the myocardial regenerative window to postnatal day 28 (P28) by a double injury pig model (ARP1MIP28) of apical resection at P1 (ARP1) and LAD ligation at P28 (MIP28). However, the molecular mechanism of regenerative window extension is not known. Results: Gene-expression profiles revealed that regenerating ARP1MIP28 hearts contained different subpopulations of cardiomyocytes early after 2nd injury. The six cardiomyocyte clusters were identified (CM1-CM6) by Louvain clustering. The CM6, CM3, and CM2 clusters have mainly consisted of fetal (92.4%), CTL-P1 (95.7%), and CTL-P56 (96.2%) cardiomyocytes. In contrast, the CM1 cluster has consisted of heterogeneous cardiomyocyte groups from all other animal groups, including less than 1% of fetal and CTL-P1 cardiomyocytes. The CM5 and CM4 clusters have mainly consisted of ARP1MIP28-P35 cardiomyocytes, 97.8%, and 68.6%, respectively. Sparse modeling analysis revealed that AR and MI injury, both alone and in combination, appeared to promote the proliferative capacity of cardiomyocytes and perturb cardiomyocyte maturation. Publication: This dataseries is associated to manuscript titled 'Single nucleus transcriptomics: Apical resection in newborn pigs extends the time-window of cardiomyocyte proliferation and myocardial regeneration', published in Circulation, 2021.
