K-562 cell lines
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1019807 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Divergence of K-562 genomes through in vitro clonal evolution revealed by comparing three sublines. Comparison of three K-562 sublines
2024-01-01 | GSE243782 | GEO
Project description:We analyzed the global effect of c-Myb knockdown by sequencing the transcriptomes of K-562 cells transfected with control siRNA and si2992 (MYB knockdown), as well as K-562 cells stably expressing TY-tagged wild type c-Myb and c-Myb D152V transfected with si2992
2017-04-24 | GSE85187 | GEO
Project description:Coemansia sp. RSA 562 Resequencing
| PRJNA581494 | ENA
Project description:To explore the mechanisms of autophagy in the regulation of vascular tumour cells, we generated three autophagy-related genes, Fip200, Atg5 and Atg7, knockout vascular tumor cells in 562 cell line by CRISPR-Cas9-based sgRNAs. Three independent replicates of mRNA samples were prepared from each KO cells and subjected to RNA-sequencing in comparison to three samples from control 562 cells. We examined changes in gene expression by transcriptional profiling of Fip200 KO, Atg5 KO, and Atg7 KO cells, compared to control 562 cells respectively.
2023-01-12 | GSE193568 | GEO
Project description:Engineered GalNAc-T glycosyltransferases were used to incorporate a chemically modified GalNAc analog into the glycoproteins on the cell surface of K-562 cells. The chemical modification included a bioorthogonal alkyne tag that allowed for introduction of a clickable, acid-cleavable biotin-picolyl-azide. Glycoproteins were enriched using Streptavidin, and on-bead digestion yielded a peptide fraction that was analysed by mass spectrometry.
2020-04-22 | PXD017989 | Pride
Project description:Single clones were isolated from the Detroit 562 cell line and implanted into NOD/SCID mice to determine whether clonal populations within one cell line had fuctionally distinct phenotypes, in the form of differing tumour initiating activity. From these data I found that different clonal populations had different tumour initiating activity, which lead to this investigation as to whether these functionally distinct populations were also genetically distinct.
2010-01-22 | GSE19994 | GEO
Project description:In a screen of airway-derived cell lines, we found several that appeared to resist SARS-CoV-2 replication despite expressing the viral receptor, ACE2. We found that innate immune activation mediated by cGAS-STING and subsequent upregulation of interferons and interferon-stimulated genes underlies this phenotype. RNA-seq was conducted on WT and STING knockout (via CRISPR) SCC25, H596, OE21 and Detroit 562 and WT Calu-3 cell lines
2024-07-22 | GSE271679 | GEO
Project description:K-562 cells were cultured in standard growth conditions and used to produce five distinct cell cultures: wild type, FHC-silenced,wild type hemin-treated, FHC-silenced hemin-treated. Then the cells were lysed and RNA was extracted using TRIzol method.Total RNA were fluorescently labelled, amplified and hybridized in triplicate for 18 hours on Illumina HumanHT12 v.4.0 Expression BeadChips and after scanning, analysis was performed with GenomeStudio v.2010.3 software, for quality control and mRNA expression analysis.
2013-10-16 | E-MTAB-1336 | biostudies-arrayexpress
Project description:A total of 432 genes were found to be differentially expressed in M1SF370 bacterial population internalized in Detroit 562 human pharyngeal cells when compared with the same strain incubated in the absence of Detroit 562 cells. While most of them (349/432 i.e. 80.8%) were up regulated, 83 genes were down regulated contributing to 19.2% of the total differentiated genes. The major contributor of the latter category was phage-related genes (35 genes). Almost ¼ of these genes (106) belonged to a category of Unknown or possible predicted function. Most notably, up-regulated genes belonged to amino acid transport , cell division, cell envelope biogenesis, DNA replication correlated well with up-regulated 67 genes belonged to translation and ribosomal structure. Further, up-regulation of 12/15 virulence-related genes indicated that human host cell internalized bacteria are highly virulent as compared to laboratory grown culture in test-tubes.
2011-06-10 | GSE27846 | GEO