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Solanum pinnatisectum

ABSTRACT: Solanum pinnatisectum ONT RNAseq and HI-C

PROVIDER: PRJNA1019935 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26213667_1.fastq.gz	Fastqsanger.gz
	SRR26213668_1.fastq.gz	Fastqsanger.gz
	SRR26213668_2.fastq.gz	Fastqsanger.gz
	SRR26213669_1.fastq.gz	Fastqsanger.gz
	SRR26213669_2.fastq.gz	Fastqsanger.gz

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Solanum pinnatisectum

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Isolation and functional characterization of the <i>SpCBF1</i> gene from <i>Solanum pinnatisectum</i>.

Project description:Low temperature causes a negative impact on plant growth and development, but plants evolve a series of mechanisms to respond to chilling stress, and one of them is CBF [C-repeat (CRT)/dehydration-responsive element (DRE) binding factor] gene family which has been well studied in different crops. In this paper, a new CBF1 gene, named as SpCBF1, was isolated from frost-tolerant Solanum pinnatisectum by PCR and analyzed for its function in cold-tolerance by over-expression technique. The ORF of SpCBF1 was 666 bp long and encoded a protein of 221 amino acids with a predicted molecular mass 24.5821 kDa and theoretically isoelectric point 5.0. SpCBF1 protein contained a highly conserved specific AP2/ERF domain. SpCBF1 was expressed in all tested tissues with the highest level in tuber and the lowest in root, and induced by chilling stress (0 °C). Under natural low temperature condition (1-10 °C), plants over-expressing SpCBF1 (OE) exhibited slighter necrotic lesion and lower necrotic injury, compared with untransformed Solanum tuberosum cv. Désirée (WT) and antisense-StCBF1 control lines. Over-expression of CBF1 increased the level of COR (cold-regulated) gene transcripts in OE lines, and the physiological indexes related to cold tolerance like the contents of SOD, soluble protein, MDA, proline and soluble sugar were higher in OE lines than in WT except RWC which was lower. All these results indicated that SpCBF1 gene plays a promoting role in potato responding to cold stress.

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Project description:Solanum lycopersicum Early Developmental Analysis Through RNAseq

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microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum

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Solanum torvum transcriptional responses to nematodes

Project description:Solanum torvum Sw is worldwide employed as rootstock for eggplant cultivation because of its vigour and resistance/tolerance to the most serious soil-borne diseasesas bacterial, fungal wilts and root-knot nematodes. A 30,0000 features custom combimatrix chip was designed and microarray hybridizations were conducted for both control and 14 dpi (day post inoculation) with Meloidogyne incognita-infected roots samples. We also tested the chip with samples from the phylogenetically-related nematode-susceptible eggplant species Solanum melongena.The genes identified from S. torvum catalogue, bearing high homology to knownnematode resistance genes, were further investigated in view of their potential role in the nematode resistance mechanism. total RNA was extracted from control and 14 days post-infection (infection with root-knot nematode Meloidogyne incognita) from roots of Solanum torvum and Solanum melongena. Three biological replicates were used for each condition and genotype for a total of 12 samples.

2013-11-01 | E-GEOD-47008 | biostudies-arrayexpress

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