Project description:We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during chronic HEV infection in human hepatocytes, the main site of HEV replication, using HepaRG cells. We have performed a preliminary screen using whole-cell RNA extracts prepared from HepaRG mock-infected or infected cells to determine whether HEV was able to trigger an IFN response. Different multiplicities of infection (MOI) (10 and 100 genome equivalent (GE)/cell) and time points (D+7, D+14, D+26, D+40, D+72 and D+100) were analyzed using the PCR array. We have also treated HepaRG cells after overnight treatment with IFN-β to confirm the ability of HepaRG to respond to IFN-I treatment.

2023-11-08 | GSE243855 | GEO

Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [D100 plaque 1]

Project description:Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [D100 plaque 1]

| PRJNA1020176 | ENA

Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [pre-array]

Project description:Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [pre-array]

| PRJNA1020171 | ENA

Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV) [pig liver]

Project description:We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during HEV infection in swine liver tissues.

2023-11-08 | GSE243862 | GEO

Cellular response to hepatitis E virus (HEV) infection

Project description:What happens in cells infected with HEV is largely unknown. We used a recently established genotype 3 HEV cell culture system and profiled the host responses by RNA-seq.

2019-10-31 | GSE88731 | GEO

Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV)

Project description:Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV)

| PRJNA1020180 | ENA

HEV

Project description:HEV blood donnors

| PRJEB24793 | ENA

Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV) [pig liver]

Project description:Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV) [pig liver]

| PRJNA1020183 | ENA

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