Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spatial RNA sequencing analysis confirm ACTC1+ myofibers support a transplanted SMPC pool

ABSTRACT: Spatial RNA sequencing analysis confirm ACTC1+ myofibers support a transplanted SMPC pool

PROVIDER: PRJNA1020199 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26152700_1.fastq.gz	Fastqsanger.gz
	SRR26152700_2.fastq.gz	Fastqsanger.gz
	SRR26152701_1.fastq.gz	Fastqsanger.gz
	SRR26152701_2.fastq.gz	Fastqsanger.gz
	SRR26152702_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 244

Similar Datasets

Spatial RNA sequencing analysis confirm ACTC1+ myofibers support a transplanted SMPC pool

Project description:Spatial transcriptomics analysis of three different experiments (describe below) identify regenerating ACTC1+ myofibers as a supportive cell type for transplanted SMPCs. Data correspond to Figures 6-7 and Extended Data Figures 8-10 in Hicks et al., "Regenerating Human Skeletal Muscle Forms an Emerging Niche In Vivo to Support PAX7 cells".

2023-09-22 | GSE243875 | GEO

Dystrophic single myofibers vs. control single myofibers

Project description:Dystrophic single myofibers vs. control single myofibers

| PRJNA125993 | ENA

Dystrophic single myofibers vs control single myofibers

Project description:Dystrophic single myofibers vs control single myofibers

| PRJNA126531 | ENA

Dystrophic single myofibers vs control single myofibers

Project description:MicroRNA-expression profile of dystrophic single fibres vs wild type single fibers isolated from different muscle of mdx and c57bl mice. Myofibers were isolated from different muscle type (tibialis, diaphragm and quadriceps of gender- (male) and age- (3 month old and half) matched wt and dystrophic mice). 9 total samples per animal model, 3 replicates per muscle type sample

2012-12-21 | E-GEOD-21219 | biostudies-arrayexpress

Dystrophic single myofibers vs. control single myofibers

Project description:MicroRNA-expression profile of dystrophic single fibers compared to wild type single fibers isolated from different muscles of mdx and C57BL mice. Myofibers were isolated from different muscle types (tibialis, diaphragm and quadriceps) of gender- (male) and age- (3 month old and half) matched wt and dystrophic mice. 9 total samples per animal model (C57BL, mdx), 3 replicates per muscle type.

2015-11-25 | E-GEOD-21498 | biostudies-arrayexpress

Dystrophic single myofibers vs control single myofibers

2012-12-21 | GSE21219 | GEO

Expression Signatures of Single Isolated Myofibers of Mouse Hindlimb.

Project description:Skeletal muscles are composed by different myofiber types with a wide range of metabolic and functional properties. In the past years, biophysical analyses on isolated muscle fibers were extensively used to study the different contractile features of each myofiber type. To better clarify the complex mechanisms determining and regulating this heterogeneity, we applied genomic technologies at the level of single isolated myofibers. Fibers were prepared from soleus and EDL mouse muscles in order to obtain a comprehensive collection of fiber types classified according to myosin heavy chain (MyHC) isoforms. Transcriptomic analysis performed using microarray technology produced expression profiles of myofibers free from the background of non-contractile cell of the muscles. This permitted to identify a complete catalogue of genes differentially expressed among fiber types, mainly coding for metabolic enzymes, isoforms of the contractile proteins, and proteins involved in calcium homeostasis. Interestingly, myofibers transcriptionally grouped in 3 different transcriptional clusters, named transcriptional slow (tS), intermediate (tI), and fast(tF), mainly according to their metabolism and speed of contraction. This transcriptionally based myofibers grouping only partially fit with myofibers classification based on MyHC isoforms. In addition, using microarray data, we identified a limited set of transcriptional markers that can be used to unequivocally classify myofibers in one of the three clusters (tS, tI, or tF). This approach based on single cell analysis would allow a better description and comprehension of the adaptive transitions at transcriptional level occurring in myofibers under physiological and pathological conditions.

2019-12-20 | GSE98325 | GEO

RNASeq of MuSCs and single myofibers

Project description:RNASeq of MuSCs and single myofibers

| PRJNA1108302 | ENA

Effect of endurance training on expression signatures of mouse type I and type IIb myofibers

Project description:Skeletal muscle myofibers, categorized into slow-twitch (type I) and fast-twitch (type II) fibers based on myosin heavy chain (MHC) isoforms, exhibit varying fatigue resistance and metabolic reliance. Type I myofibers are fatigue-resistant with high mitochondrial density and oxidative metabolism, while Type II myofibers fatigue quickly due to glycolytic metabolism and fewer mitochondria. Endurance training induces remodeling of myofiber and mitochondrial, increasing slow-twitch myofibers and enhancing mitochondrial oxidative capacity, improving muscle fitness. In our study, conducted using single-cell techniques, we delved deeply into the transcriptomic differences between type I and type IIb myofibers. In response to endurance training, type I myofibers exhibited heightened signals in essential adaptive responses, such as fatty acid oxidation, mitochondrial biogenesis, and protein synthesis, compared to type IIb myofibers. By analyzing untrained myofibers, we identified specific signaling pathways that explain the differences in their responses to endurance training. These findings provide nuanced insights into the molecular mechanisms governing endurance adaptations in fast and slow-twitch muscles, offering valuable guidance for tailored exercise routines and potential therapeutic interventions.

2024-12-31 | GSE244547 | GEO

Subcellular localization analysis of long non-non coding RNAs (lncRNAs) in skeletal muscle myofibers

Project description:In order to study the subcellular localization of lncRNAs in myofibers of skeletal muscle, single myofibers were isolated from Soleus, Extensor Digitorum Longus (EDL) and Tibialis Anterior (TA) of 9 mice. RNA was extracted from nuclei and cytoplasmic fractions of 5-10 isolated myofibers and than separately hybridized on microarrays. Preferential expression in nuclear or cytoplasmic compartment of each lncRNA was determined.

2019-01-07 | GSE112768 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data