Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Modeling pre-implantation development using totipotent blastomere-like stem cells [Cut&tag]

ABSTRACT: Modeling pre-implantation development using totipotent blastomere-like stem cells [Cut&tag]

PROVIDER: PRJNA1020576 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Modeling pre-implantation development using totipotent blastomere-like stem cells

Project description:Modeling pre-implantation development using totipotent blastomere-like stem cells

| PRJNA1020571 | ENA

Modeling pre-implantation development using totipotent blastomere-like stem cells [ATAC-seq]

Project description:Modeling pre-implantation development using totipotent blastomere-like stem cells [ATAC-seq]

| PRJNA1020575 | ENA

Modeling pre-implantation development using totipotent blastomere-like stem cells [scRNA-seq]

Project description:Modeling pre-implantation development using totipotent blastomere-like stem cells [scRNA-seq]

| PRJNA1020574 | ENA

Modeling pre-implantation development using totipotent blastomere-like stem cells [Cut&tag]

Project description:we have established TBLC-based differentiation systems to recapitulate the entire pre-implantation development Cleavage under target & tagmentation (CUT&Tag)-sequencing (CUT&Tag) for H3K4me3,H3K27ac,H3K9me3,RNA polymerase II(Pol2) and h3k27me3 in mPSC sand mTBLCs

2025-01-17 | GSE243923 | GEO

Modeling pre-implantation development using totipotent blastomere-like stem cells [bulk RNA-seq]

Project description:Modeling pre-implantation development using totipotent blastomere-like stem cells [bulk RNA-seq]

| PRJNA1020573 | ENA

Remodeling the step-wise mouse placental development by using totipotent blastomere-like stem cells [CUT&Tag]

Project description:During development, totipotent blastomeres initiate the first cell fate decision to generate inner cell mass and trophectoderm. The trophectoderm forms the placenta mediating fetal-maternal communications, while placental deficiencies cause infertility and pregnancy disorders in humans. However, in vitro systems remodeling the step-wise placental development, particularly encompassing the pre-implantation phase, are still unavailable. Here, using mouse totipotent blastomere-like cells (TBLCs), we successfully realize inducing and long-term maintaining trophectoderm-like stem cells (TELSCs), and further generating placental trophoblast organoids. Interestingly, an intermediate morula trophectoderm-like cells (TELCs) were transiently induced from TBLCs, and quickly converted into TELSCs assembling blastocyst trophectoderm. In 3D culturing condition, TELSCs can form rosette-like structures at peri-implantation period, to eventually generate trophoblast organoids, in which trophoblast progenitors/giant cells, spongiotrophoblasts and syncytiotrophoblasts were all identified at the single-cell level. Transcriptomic and epigenomic analyses enable tracing the step-by-step transition from TBLCs to mature trophoblast lineages. Thus, this study provides a comprehensive differentiation system to understand and investigate placental development.

2025-01-01 | GSE251981 | GEO

Highly efficient generation of blastocyst-like structures from mouse totipotent blastomere-like cells

Project description:Mammalian embryogenesis begins with a totipotent zygote. Early embryogenesis can be recapitulated by aggregated extended pluripotent stem cells (EPSCs) in a 3D culture system. However, the efficiency of generating blastoids is low and whether other reported totipotent stem cells retained a similar capacity remains unknown. Here we show that spliceosomal repressed totipotent blastomere-like cells (TBLCs) form blastocyst-like structures when aggregated in 3D microwells with around 80% efficiency. TBLC-blastoids resemble blastocysts in morphology and cell-lineage allocation, show similar transcriptional profile with natural blastocysts and contain more TE cells and fewer undefined intermediate cells compared to blastoids from EPSCs. Moreover, TBLC-blastoids can develop beyond the implantation stage in vitro and induce decidualization after transferred into uterus. In summary, we supply an alternative cell type to generate ameliorated blastoids highly efficiently for studying early mouse embryogenesis.

2022-11-08 | GSE197779 | GEO

Modeling pre-implantation development using totipotent blastomere-like stem cells [scRNA-seq]

Project description:we have established TBLC-based differentiation systems to recapitulate the entire pre-implantation development

2025-01-17 | GSE243922 | GEO

Modeling pre-implantation development using totipotent blastomere-like stem cells [ATAC-seq]

Project description:we have established TBLC-based differentiation systems to recapitulate the entire pre-implantation development Assay for transposase accessible chromatin with high-throughput sequencing in mESC sand mTBLCs

2025-01-17 | GSE243924 | GEO