Project description:Gluconacetobacter diazotrophicus has been the focus of several studies aiming to understand the mechanisms behind this endophytic diazotrophic bacterium. The present study is the first global analysis of the early transcriptional response of exponentially growing G. diazotrophicus to iron, an essential cofactor for many enzymes involved in various metabolic pathways. RNA-seq, targeted gene mutagenesis and computational motif discovery tools were used to define the G. diazotrophicus Fur regulon. The data analysis showed that genes encoding functions related to iron homeostasis, were significantly upregulated in response to iron limitation. Certain genes involved in the secondary metabolism were overexpressed under iron-limited conditions. In contrast, it was observed that the expression of genes involved in Fe-S cluster biosynthesis, flagellar biosynthesis and type IV secretion systems were downregulated in an iron-depleted culture medium. Our results support a model that control transcription in G. diazotrophicus by Fur function. The G. diazotrophicus Fur protein was able to complement an E. coli fur mutant. These results provide new insights into the effects of iron on the metabolism of G. diazotrophicus, as well as demonstrating the essentiality of this micronutrient for the main characteristic of plant growth promotion by G. diazotrophicus.

2021-01-09 | GSE164445 | GEO

Vibrio diazotrophicus strain:65.10M

Project description:Vibrio diazotrophicus strain:65.10M Genome sequencing and assembly

| PRJNA429233 | ENA

Vibrio diazotrophicus strain:60.18M

Project description:Vibrio diazotrophicus strain:60.18M Genome sequencing and assembly

| PRJNA429138 | ENA

Oharaeibacter diazotrophicus strain:SM30

Project description:Oharaeibacter diazotrophicus strain:SM30 Genome sequencing and assembly

| PRJNA597415 | ENA

Pontibacter diazotrophicus strain:H4X

Project description:Pontibacter diazotrophicus strain:H4X Genome sequencing and assembly