Project description:To profile genome-wide allele-specific expression in an unbiased manner we designed a high-resolution yeast tiling microarray that covers the genomes of both the laboratory strain S288c and the recently sequenced clinical isolate YJM789. This array design allows simultaneous expression profiling of allelic variants in a heterozygous hybrid strain. We hybridized cDNA from the heterozygous Y/S and from the homozygous S and Y strains grown in rich medium (YPD). Strand specificity during sample preparation was maintained by inclusion of actinomycin D during reverse transcription to prevent spurious synthesis of second strand cDNA.

2009-02-10 | E-TABM-569 | biostudies-arrayexpress

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