Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals

ABSTRACT: Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals

PROVIDER: PRJNA1021638 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26196917.fastq.gz	Fastqsanger.gz
	SRR26196918.fastq.gz	Fastqsanger.gz
	SRR26196919.fastq.gz	Fastqsanger.gz
	SRR26196920.fastq.gz	Fastqsanger.gz
	SRR26196921.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 17

Similar Datasets

Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals [ChIP-seq]

Project description:Members of the diverse heterochromatin protein 1 (HP1) family of proteins play crucial roles in heterochromatin formation and maintenance. Despite the similar affinities of their chromodomains for di- and tri-methylated histone H3 lysine 9 (H3K9me2/3), different HP1 proteins exhibit distinct chromatin binding patterns, presumably due to their interactions with various specificity factors. Here, we elucidate the molecular basis of the protein-protein interaction between the HP1 protein Rhino, a critical factor of the Drosophila piRNA pathway, and Kipferl, a DNA sequence-specific C2H2 zinc finger protein and Rhino guidance factor. Through phylogenetic analyses, structure prediction, and in vivo genetics, we identify a single amino acid change within Rhino’s chromodomain, G31D, that does not affect H3K9me2/3 binding but abolishes the specific interaction between Rhino and Kipferl. Flies carrying the rhinoG31D mutation phenocopy kipferl mutant flies, with Rhino redistributing from piRNA clusters to satellite repeats, causing pronounced changes in the ovarian piRNA profile of rhinoG31D flies. Thus, Rhino’s chromodomain serves as a dual-specificity module, facilitating interactions with both a histone mark and a DNA-binding protein.

2023-10-03 | GSE244192 | GEO

Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals [RNA-seq]

2023-10-03 | GSE244195 | GEO

Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals [RNA-seq]

Project description:Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals [RNA-seq]

| PRJNA1021641 | ENA

Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals [ChIP-seq]

Project description:Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals [ChIP-seq]

| PRJNA1021636 | ENA

Evolutionary adaptation of the chromodomain of the HP1 protein Rhino allows th eintegration of heterochromatin and DNA sequence signals

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-10-03 | GSE244196 | GEO

The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters (ChIP-Seq)

Project description:RNA interference systems depend on the synthesis of small RNA precursors whose sequences define the target spectrum of these silencing pathways. The Drosophila Heterochromatin Protein 1 (HP1) variant Rhino permits transcription of PIWI-interacting RNA (piRNA) precursors within transposon-rich heterochromatic loci in germline cells. Current models propose that Rhino’s specific chromatin occupancy at piRNA source loci is determined by histone marks and maternally inherited piRNAs, but also imply the existence of other, undiscovered specificity cues. Here, we identify a member of the diverse family of zinc finger associated domain (ZAD)-C2H2 proteins, Kipferl, as critical Rhino cofactor in ovaries. By binding to guanosine-rich DNA motifs and interacting with the Rhino chromodomain, Kipferl recruits Rhino to specific loci and stabilizes it on chromatin. In kipferl mutant flies, Rhino is lost from most of its target chromatin loci and instead accumulates on pericentromeric satellite arrays, resulting in decreased levels of transposon targeting piRNAs and impaired fertility. Our findings reveal that DNA sequence, in addition to the H3K9me3 mark, determines the identity of piRNA source loci and provide insight into how Rhino might be caught in the crossfire of genetic conflicts.

2022-05-15 | GSE202465 | GEO

The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters (sRNA-Seq)

2022-05-15 | GSE202467 | GEO

The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters (RNA-Seq)

2022-05-15 | GSE202466 | GEO

Trans-generationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing [Rhino RNA-seq]

Project description:Small non-coding RNAs that associate with Piwi proteins, called piRNAs, serve as guides for repression of diverse transposable elements in germ cells of Metazoa. In Drosophila, the genomic regions that give rise to piRNAs, the so-called piRNA clusters, are transcribed to generate long precursor molecules that are processed into mature piRNAs. How genomic regions that give rise to piRNA precursor transcripts are differentiated from the rest of the genome and how these transcripts are specifically channeled into the piRNA biogenesis pathway are not known. We found that trans-generationally inherited piRNAs provide the critical trigger for piRNA production from homologous genomic regions in the next generation by two different mechanisms. First, inherited piRNAs enhance processing of homologous transcripts into mature piRNAs by initiating the ping-pong cycle in the cytoplasm. Second, inherited piRNAs induce installment of the H3K9me3 mark on genomic piRNA cluster sequences. The HP1 homolog Rhino binds to the H3K9me3 mark through its chromodomain and is enriched over piRNA clusters. Rhino recruits the piRNA biogenesis factor Cutoff to piRNA clusters and is required for efficient transcription of piRNA precursors. We propose that trans-generationally inherited piRNAs act as an epigenetic memory for identification of substrates for piRNA biogenesis on two levels, by inducing a permissive chromatin environment for piRNA precursor synthesis and by enhancing processing of these precursors. total RNA sequencing of RNA from ovaries of shWhite and Rhino knockdown flies

2014-07-26 | E-GEOD-59607 | biostudies-arrayexpress

HP1

Project description:HP1

| PRJNA326779 | ENA