Project description:The isabelline wheatear Oenanthe isabellina was widespread in Northern Hemisphere, but the information of this species is poorly known. In this study, the complete sequence of O. isabellina mitochondrial genome was obtained by L-PCR and conserved primer-walking approaches. The results showed that the entire mitochondrial genome of O. isabellina was 16,812?bp in length with 52.7% A?+?T content; the genome harbored the same gene order as that of other birds, including 2 rRNA genes, 22 tRNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop). All protein-coding genes of O. isabellina mitochondrial genome started with an ATG codon, except for COI with GTG. For terminate codon usage, most of the genes use TAA or TAG. The control region of O. isabellina was located between tRNA-Glu and tRNA-Phe with 1244?bp length, no repetitive sequence. The mitochondrial data are potentially important for understanding this poorly known species.