Project description:We performed that comprehensive identification of genes responsible for stress tolerance by analyzing the whole-genome expression profiles of poplar (Populus alba × P. glandulosa) leaves exposed to drought and salt stresses. Examination at the molecular level how this tree species responds to drought and salt stresses by regulating the expression of genes involved in signal transduction, transcriptional regulation, and stress responses.
Project description:Here we applied a novel approach to isolate nuclei from complex plant tissues (https://doi.org/10.1371/journal.pone.0251149), to dissect the transcriptome profiling of the hybrid poplar (Populus tremula × alba) vegetative shoot apex at single-cell resolution.
Project description:Transcriptome analysis was performed on the sixth leaves of Populus alba × P. glandulosa. The twelve samples were: four PagSUPa overexpression (OE) line 2 samples 2-1-L6, 2-2-L6, 2-3-L6 and 2-4-L6, four PagSUPa OE line 7 samples 7-1-L6, 7-2-L6, 7-3-L6 and 7-4-L6, four nontransgenic control (CK) samples CK-1-L6, CK-2-L6, CK-3-L6, CK-4-L6 . And the differential genes between PagSUPa OE and CK groups were identified and compared, which helps to reveal the molecular mechanism of PagSUPa directly suppresses the expression of phragmoplast orienting and positioning genes PagPOK1 and PagPOK2, and impairs cytokinesis and cell wall organization.
Project description:Illumina technology was used to generate mRNA profiles of Populus tremula x alba 717-1B4 control roots and Laccaria bicolor S238N ectomycorrhiza. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the Populus trichocarpa v4.1 primary transcripts available at Phytozome (https://phytozome-next.jgi.doe.gov/info/Ptrichocarpa_v4_1l) using CLC Genomics Workbench v24.
