Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1027998 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR26384683_1.fastq.gz | Fastqsanger.gz | |||
| SRR26384683_2.fastq.gz | Fastqsanger.gz | |||
| SRR26384684_1.fastq.gz | Fastqsanger.gz | |||
| SRR26384684_2.fastq.gz | Fastqsanger.gz | |||
| SRR26384685_1.fastq.gz | Fastqsanger.gz |
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Project description:Lion-head goose is the only large goose species in China, and it was one of the largest goose species in the world. Our previous study firstly reported a chromosome-level genome assembly of Lion-head goose (Anser cygnoides), a native breed in South China, through the combination of PacBio, Bionano, and Hi-C technologies. The fat content of foie gras is augmented during its preparation due to the special feeding regimen. Lion-head geese have a strong tolerance of massive energy intake and show a priority of fat accumulation in liver tissue. In this study, we studied for the first time the important differential genes that regulate fatty liver in Lion-head goose. After high-intake feeding, the fatty livers of Lion-head geese were distinctly characterized. The revelation of gene regulation is an important basis for the study of liver development and molecular characteristics for the Lion-head goose. To analyze the excellent fatty liver performance of Lion-head goose at the molecular level, we performed whole transcriptome analysis by high-throughput RNA sequencing to analyze the key regulatory genes that determine the fatty livers in high-intake feeding group compared with the normal livers in normally-fed Lion-head geese. We identified 716 differentially expressed mRNAs, 145 differentially expressed circRNAs, and 39 differentially expressed lncRNAs in the fatty livers in high-intake feeding group compared with the normal livers in normally-fed Lion-head geese, including upregulated and downregulated genes, respectively. GO enrichment analysis showed that these genes were significantly enriched in molecular function, involved in extracellular regions, DNA-binding transcription factor activity, extracellular matrix, heme binding and other life activities. We chose differentially expressed genes involved in either upregulation or downregulation, and we additionally confirmed the accuracy of sequencing at the RNA level. In summary, our research suggested that these differentially expressed genes may play important roles in fatty liver development in Lion-head goose. However, the functions and mechanisms of these significantly differentially expressed genes should be investigated in future studies.
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Project description:Introduction: Emerging proof suggests that Apocynum venetum flowers polysaccharides (AVFP) has immunomodulatory effects in vitro. However, the action mechanism of AVFP is still unclear in vivo. The purpose of this study is to probe into the potential mechanism of AVFP on immunosuppressed mice through investigating organ index, cytokine levels, anti-oxidative stress capacity, transcriptomics and gut microbiota. Methods: Immunocompromised mouse model induced by cyclophosphamide (CXT) was divided into 6 groups. Enzyme-labeled method, HE, transcriptomics and high-throughput sequencing were used to detect the regulatory effects of AVFP on immunocompromised mice, as well as the function of AVFP on the concentration of short-chain fatty acids (SCFAs) by high-performance liquid chromatographic analysis (HPLC). Pearson was used to analyze the correlation between intestinal microbiota and biochemical indexes. Results: The experimental results illustrated that AVFP has protective effects against CXT-induced immunosuppression in mice via prominently increasing organ index, levels of anti-inflammatory factors in serum as well as enhancing antioxidant capacity of the liver. Meanwhile, it could also signally decrease the level of pro-inflammatory cytokines in serum, the activity of transaminase in serum and the content of free radicals in liver. Transcriptomics results discovered that AVFP could play a role in immune regulation via participating in NF-κB signaling pathway, regulating the immune-related genes Bcl3, Hp, Lbp, Cebpd, Gstp2 and Lcn2. Intestinal microbiota results illustrated that AVFP could increase abundance of beneficial bacteria, reduce abundance of harmful bacteria, while dramatically improved the content of SCFAs, modulated immune responses and improved host metabolism. Spearman analysis further assessed the correlation between gut microbiota and biochemical indicators. Conclusion: These findings demonstrated that AVFP could enhance the immune effects of the immunosuppressed mice and improve the body's ability to resist oxidative stress.
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