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ABSTRACT: Rehydration under extreme drought conditions affected rhizosphere soil microorganisms more than bulk soil in broomcorn millet farmland

PROVIDER: PRJNA1028307 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26408030_1.fastq.gz	Fastqsanger.gz
	SRR26408030_2.fastq.gz	Fastqsanger.gz
	SRR26408031_1.fastq.gz	Fastqsanger.gz
	SRR26408031_2.fastq.gz	Fastqsanger.gz
	SRR26408032_1.fastq.gz	Fastqsanger.gz

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Project description:Rehydration under extreme drought conditions affected rhizosphere soil miicroorganisms more than bulk soil in broomcorn millet farmland

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RNAseq of drought treated Populus trichocarpa

Project description:To identify genes that are drought-responsive we conducted drought (soil water depletion) experiments on 3-month-old *P*. *trichocarpa *clonal plants. The plants undergo five different stages based on the appearance of their shoots and leaves during the drought experiments. Stage I: The shoot and leaves are green, and the leaves are well-spread. Stage II: The leaves are droopy. Stage III: The shoot is droopy, and the leaves are partially dry. Stage IV: The leaves are brown and totally dry. Stage V: The shoot is brown. With fully irrigation, the soil water content is 74% and the xylem water content is 80.6%. Plants in Stage III (Day 5) are under a mild drought state. The soil and xylem water content in Stage III dropped to 33% and 75.3%, respectively. Stage IV (Day 6-10) is a severe drought state where the soil and xylem water content continued decreasing to 29% and 74.3%, respectively in Day 7. The stressed plants from Stage I-IV could all recover in 3 days after rehydration, but the plants in Stage V could not recover after rehydration.

2018-12-27 | GSE79401 | GEO

Project description:Transcriptome response to drought in broomcorn millet

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Project description:the rhizosphere microorganisms of Broomcorn millet (Panicum miliaceum L.)

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Project description:the rhizosphere microorganisms of Broomcorn millet (Panicum miliaceum L.)

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millet leaf and farmland soil

Project description:millet leaf and farmland soil Metagenome

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Project description:Resequencing of broomcorn millet

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Comprehensive analysis of rice transcriptome during dehydration and rehydration

Project description:Drought is a harmful abiotic stress that threatens the growth, development, and yield of rice plants. To cope with drought stress, plants have evolved diverse and sophisticated stress-tolerance pathways by regulating gene expression. Previous genome-wide studies have revealed many rice drought stress-responsive genes that are involved in various metabolism, hormone biosynthesis and signaling pathways, and transcriptional regulation. However, little is known about the regulation of drought-responsive genes during rehydration after dehydration. In this study, we examined the dynamic gene expression patterns in rice seedling shoots during dehydration and rehydration using RNA-seq analysis. To investigate the transcriptome-wide rice gene expression patterns during dehydration and rehydration, RNA-seq libraries were sequenced and analyzed to identify differentially expressed genes (DEGs). DEGs were classified into five clusters based on their gene expression patterns. The clusters included drought-responsive DEGs that were either rapidly or slowly recovered to control levels by rehydration treatment. Representative DEGs were selected and validated using qRT-PCR. In addition, we performed a detailed analysis of DEGs involved in nitrogen metabolism, phytohormone signaling, and transcriptional regulation. In this study, we revealed that drought-responsive genes were dynamically regulated during rehydration. Moreover, our data showed the potential role of nitrogen metabolism and jasmonic acid signaling during the drought stress response. The transcriptome data in this study could be a useful resource for understanding drought stress responses in rice, and may provide a valuable gene list for developing drought-resistant crop plants.

2023-05-17 | GSE222438 | GEO

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