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ABSTRACT:
PROVIDER: PRJNA1028488 | ENA |
REPOSITORIES: ENA
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Project description:The shrimp aquaculture industry is vulnerable to large losses due to acute hepatopancreatic necrosis disease (AHPND), caused by the bacterium Vibrio parahaemolyticus. The mechanism by which the pathogen causes disease, and the host immune response, is not completely understood. The shrimp hepatopancreas is a multi-functional organ with roles in digestion, immunity, molting and reproduction. Therefore, we set out to characterize the cells of the hepatopancreas and the host response to Vibrio parahaemolyticus infection at single-cell resolution. The hepatopancreas from three individual shrimp were processed to create a single-cell transcriptomic atlas. Then the hepatopancreas from three Vibrio parahaemolyticus infected and two mocked treated shrimp were sampled for infection study. All single-cell libraries were generated using the 10X Genomics platform and sequenced on an Illumina sequencer. Data were aligned to the Litopenaeus vannamei reference genome using Cell Ranger. Seurat and clusterProfiler were used for downstream analyses. Cells of the hepatopancreas were characterized and the transcriptomic response to AHPND-causing V. parahaemolyticus was examined. Data will inform further functional studies and has the potential to aid in the development of novel preventative measures or treatments.
2026-02-18 | GSE306674 | GEO
Project description:Vibrio species are recognized for their role in food- and water-borne diseases in humans, fish, and aquatic invertebrates. We screened bacterial strains isolated from raw food shrimp for those that are bactericidal to Vibrio strains. Here we identify and characterize Aeromonas dhakensis strain A603 which shows robust bactericidal activity specifically towards Vibrio and related taxa but less potency toward other Gram-negative species. Using the A603 genome and genetic analysis, we show that two antibacterial mechanisms account for its vibriocidal activity -- a highly potent Type Six Secretion System (T6SS) and biosynthesis of a vibriocidal phenazine-like small molecule, named here as Ad-Phen. Further analysis indicates coregulation between Ad-Phen and a pore-forming T6SS effector TseC, which potentiates V. cholerae to killing by Ad-Phen.
2024-07-05 | GSE270023 | GEO
Project description:Maintaining marine salinities in inland aquaculture systems can be cost-prohibitive. As such, there has been an emphasis on the identification of marine species that can be reared at low salinities. The Florida pompano (Trachinotus carolinus) is a popular sportfish that can survive in salinities as low as 5 ppt and has been identified as a potential target species for commercial production. Vibrio spp., bacterial pathogens ubiquitous in aquaculture systems, are capable of quickly decimating aquaculture populations. However, there is limited data available on the effects of salinity influences on Vibrio susceptibility in juvenile Florida pompano. This study investigated the influence of lowered salinity on the immune response of juvenile Florida pompano exposed to Vibrio across a 14-day period through assessment of transcriptomic changes, fatty acid biosynthesis, and identification of internal and external signs of vibriosis. During the experimental period, there were no differences in osmoregulatory or immune related gene expression, no obvious signs of vibriosis, and no changes in fatty acid biosynthesis in fish exposed to 8, 12, 20, or 28 ppt seawater. These results suggest juvenile Florida pompano are resilient to salinity changes and exhibit baseline molecular machinery capable of responding to pathogenic challenges across a range of salinities, making them strong candidates for rearing in inland aquaculture.
2026-08-01 | GSE248270 | GEO
Project description:World aquaculture production of the Pacific white shrimp (Litopenaeus vannamei) is estimated to account for 80% of the total shrimp produce worldwide. The global demand for shrimp has driven the industry to utilize and rely on semi-intensive and intensive shrimp systems. In the United States, Pacific white shrimp production can take place in semi-intensive earthen ponds, recirculating aquaculture systems (RAS), biofloc technology and green water. In this study, the effects of lowering dissolved oxygen conditions in outdoor green water tanks on global gene expression is examined. Tissue samples from the gill and intestine were collected for gene expression analysis via RNA sequencing. Among all comparisons, RNA sequencing revealed the up-regulation of a single gene: hydroxyacid oxidase 1 gene. The HOA1 gene was found to be 7-fold higher in the intestine sample at the medium aeration level compare to that of the high (control) level. The HAO1 gene, also known as glycolate oxidase 1 (GOX1) is a gene related to the 2-hydroxyacid oxidase enzyme that is part of the oxidoreductase family and plays a role in glyoxylate and dicarboxylate metabolism. The identification of a single differentially expressed gene across all analyzed samples suggests that Pacific white shrimp exposed to lowering dissolved oxygen set points does not induce global changes in gene expression at these levels.
2025-07-17 | GSE281217 | GEO
Project description:Vibrio harveyi is an important pathogen in aquaculture, which can cause severe organ damage and metabolic disorders in fish. The organ damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.
2025-08-30 | E-MTAB-15492 | biostudies-arrayexpress
Project description:Vibrio harveyi is an important pathogen in aquaculture, which can cause severe intestinal damage and metabolic disorders in fish. The intestinal damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.
2025-10-25 | E-MTAB-15691 | biostudies-arrayexpress
Project description:The phenomenon of trained immunity, which facilitates vaccine development for disease control, has been identified in shrimp; however, the mechanism remains elusive. In the present study, we found that histone H3K27 acetylation (H3K27ac) mediated by the lysine acetyltransferase KAT8 plays an important role in preventing white spot syndrome virus (WSSV) infection in the shrimp Marsupenaeus japonicus. We then successfully established a model of trained immunity via the use of UV-inactivated WSSV to explore the underlying mechanism(s) in shrimp. In UV-WSSV-trained shrimp, the glycolysis and tricarboxylic acid (TCA) cycle metabolic pathways were enhanced and acetyl-CoA concentrations were increased. As the acetyl group donor, acetyl-CoA promotes KAT8 activity to increase H3K27 acetylation. H3K27ac is deposited at the promoter region of the transcription factor Dorsal to facilitate its expression and then Dorsal promotes the expression of an interferon-like cytokine, Vago5, and antimicrobial peptides that act against WSSV infection. H3K27ac is also deposited at the promoter region of hexokinase 2 and isocitrate dehydrogenase, which positively regulates glycolysis and the TCA cycle in a feedforward manner. Our results reveal a novel mechanism of trained immunity induced by UV-WSSV in shrimp and provide a theoretical basis for the development of antiviral vaccines for disease control in shrimp aquaculture.
2025-08-01 | GSE273689 | GEO