Project description:Transcriptomic analysis revealed differences in gene expression between control and HSF1 knockout cells, revealing how HSF1 gene regulation may be altered when knocked out.
Project description:Heat shock transcription factor 1(HSF1) is an important transcription factor which regulates the expression of a wide array of genes including heat shock proteins and oncogenes. Here, we report that HSF1 as a target of WNT/β-catenin signaling, regulates parts of target genes of WNT/β-catenin signaling. To explore the biological relevance of HSF1 activation to WNT/β-catenin signaling, we profiled gene expression of wild type mouse embryonic fibroblasts (WT MEF) and HSF1 knock out MEF (HSF1 KO MEF) before and after lithium chloride (LiCl) treatment which was a potent GSK3β inhibitor and increased the expression of β-catenin.
Project description:This is a RNA-Seq analysis aiming to study the transcriptomic changes of C4-2 prostate cancer cells induced by HSF1 inhibitor DTHIB (E12)
Project description:Upon heat shock, Hsf1 protein is extensively phosphorylated, however, this modification is inhibited by an hsf1-bal mutation. To get genome-wide effect of the hyperphosphorylation on Hsf1-regulated transcription, the heat-induced gene expression profiles in HSF1 and hsf1-bal cells were compared. Keywords: stress response
Project description:To examine whether human HSF1, chicken HSF1 and chicken HSF3 regulates the expression of different genes, we performed DNA microarray analysis.