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Transcriptomic Analysis Identifies Dysregulated Aortic Ceramide Metabolism in a Miniature Pig Model of Atherosclerosis

ABSTRACT: Transcriptomic Analysis Identifies Dysregulated Aortic Ceramide Metabolism in a Miniature Pig Model of Atherosclerosis

PROVIDER: PRJNA1028786 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26403100_1.fastq.gz	Fastqsanger.gz
	SRR26403100_2.fastq.gz	Fastqsanger.gz
	SRR26403101_1.fastq.gz	Fastqsanger.gz
	SRR26403101_2.fastq.gz	Fastqsanger.gz
	SRR26403102_1.fastq.gz	Fastqsanger.gz

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Transcriptomic Analysis Identifies Dysregulated Aortic Ceramide Metabolism in a Miniature Pig Model of Atherosclerosis

Project description:Dysregulation of lipid metabolism is the pathological basis of the occurrence and development of atherosclerosis.The pig is an ideal animal to study human cardiovascular diseases. However, the specific changes of lipid metabolism in its atherosclerotic plaques, as well as the underlying mechanisms, remain to be elucidated. To investigate the alterations of lipid metabolism related pathways, especially ceramide synthesis related genes, in the abdominal aorta of atherosclerotic pigs induced by high-fat, high-cholesterol, and high-fructose (HFCF) diet, we performed RNA-Seq on abdominal aorta tissue from Wuzhishan pigs fed either normal chow diet or HFCF diet for six months.

2024-01-31 | GSE245530 | GEO

Rewiring endothelial cell sphingolipid metabolism to favor S1P over ceramide signaling protects from coronary atherosclerosis

Project description:Growing evidence correlated changes in bioactive sphingolipids, particularly sphingosine-1-phosphate (S1P) and ceramides, with coronary artery diseases. Furthermore, specific plasma ceramide species can predict major cardiovascular events. Dysfunction of the endothelium lining lesion-prone areas plays a pivotal role in the initiation and progression of atherosclerosis. Yet, how sphingolipid metabolism and signaling change and contribute to endothelial dysfunction and atherosclerosis remain poorly understood. By using a mouse model of coronary atherosclerosis, we demonstrated that hemodynamic stress induces an early metabolic rewiring of endothelial sphingolipid de novo biosynthesis favoring S1P signaling over ceramide as protective response. Furthermore, our data are paradigm shift from the current believe that ceramide accrual contributes to endothelial dysfunction. The de novo biosynthesis of sphingolipids is commenced by serine palmitoyltransferase (SPT), and is downregulated by NOGO-B, an ER membrane protein. We showed that Nogo-B is upregulated by hemodynamic stress in myocardial endothelial cells (EC) of ApoE-/- mice and is expressed in the endothelium lining coronary lesions in mice and human. We demonstrated that mice lacking Nogo-B specifically in EC (Nogo-A/BECKOApoE-/-) were resistant to coronary atherosclerosis development and progression, and mortality. Fibrous cap thickness was significantly increased in Nogo-A/BECKOApoE-/- mice and correlated with reduced necrotic core and macrophage infiltration. Mechanistically, the deletion of Nogo-B in EC sustained the rewiring of sphingolipid metabolism towards S1P, imparting an atheroprotective transcriptional signature that refrain coronary atherogenesis and its progression. These findings also set forth the foundation for sphingolipid-based therapeutics to reduce the treat this condition.

2024-02-12 | GSE254426 | GEO

Single cell RNA sequencing of aortic CD45+ cells reveals aortic macrophage heterogeneity in mouse atherosclerosis

Project description:We have applied single cell RNA sequencing (scRNA-seq) as an unbiased transcriptomics to characterize aortic immune cells in atherosclerosis. The scRNA-seq analysis revealed macrophage heterogeneity at the single-cell level in mouse atherosclerotic aorta.

2020-09-04 | GSE154692 | GEO

Microarray gene expression profiling of aortic genes of APOE-deficient mice receiving atherosclerosis treatment with the antioxidant vitamin E

Project description:Hypercholesterolemic APOE-deficient mice are a widely used experimental model of atherosclerosis and increased generation of reactive oxygen species (ROS) is a prominent feature of atherosclerosis development. To study the impact of ROS on atherogenesis, we treated APOE-deficient mice for 7 months with the antioxidant vitamin E (2000 IU/kg diet) and performed whole genome microarray gene expression profiling of aortic genes. Microarray gene expression profiling was performed of whole aortas isolated from vitamin E-treated APOE-deficient relative to untreated APOE-deficient mice with overt atherosclerosis, and nontransgenic B6 control mice. Microarray gene expression profiling revealed that vitamin E treatment prevented atherosclerosis-related gene expression changes of the aortic intima and media. Microarray gene expression profiling was performed of whole aortas isolated from APOE-deficient mice with atherosclerosis relative to vitamin E-treated APOE-deficient mice, and nontransgenic B6 control mice. Three study groups were analyzed, i.e. 8 months-old untreated APOE-deficient mice with overt atherosclerosis, age-matched APOE-deficient mice treated for 7 months with the antioxidant vitamin E (2000 IU/kd diet), and nontransgenic B6 control (C57BL/6J) mice. Two biological replicates were made of each group, and total RNA of three aortas was pooled for one gene chip. The study complements microarray study GSE19286.

2012-12-11 | E-GEOD-42813 | biostudies-arrayexpress

Single-cell RNA-seq reveals the transcriptional landscape and heterogeneity of aortic macrophages in murine atherosclerosis

Project description:We have applied single-cell RNA sequencing as an unbiased profiling strategy to interrogate and classify aortic macrophage heterogeneity at the single-cell level in atherosclerosis.

2018-03-29 | GSE97310 | GEO

Microarray gene expression profiling of aortic genes of APOE-deficient mice receiving atherosclerosis treatment with the antioxidant vitamin E

2012-12-11 | GSE42813 | GEO

Single cell RNA sequencing of aortic CD45+ cells reveals aortic macrophage heterogeneity in mouse atherosclerosis

Project description:Single cell RNA sequencing of aortic CD45+ cells reveals aortic macrophage heterogeneity in mouse atherosclerosis

| PRJNA647207 | ENA

Glutamine supplementation alleviated aortic atherosclerosis in mice model and in vitro

Project description:This study aimed to clarify the role of glutamine in atherosclerosis and its participating mechanism. Forty C57BL/6J mice were divided into wild control, ApoE-/- control, glutamine+ApoE-/-control, ApoE-/- high fat diet (HFD) and glutamine+ ApoE-/- HFD groups. The degree of atherosclerosis; plasma biochemistry and protein profiles, and western blotting and untargeted metabolomics of aortic tissue were detected at 18 weeks. An in vitro study was also performed. Glutamine treatment significantly decreased the degree of aortic atherosclerosis compared with that in the ApoE-/- HFD group (p=0.03). O-GlcNAc, IL-1β, IL-1α, GLUT1 and PKM2 in the ApoE-/- HFD group were significantly higher than those in the ApoE-/- control group (p < 0.05). These differences were reversed by glutamine treatment (p < 0.05), and aggravated by OGT overexpression in the in vitro study (p < 0.05). Multiomics showed that the ApoE-/- HFD group had higher levels of oxidative stress regulatory molecules (GUAD, XDH, PSB1 and PSB4), proinflammatory regulatory molecules (myristic acid, HMGB3, vinculin, DNJC2 and SPRC) and stress granules regulatory molecules (caprin-1 and DEAR) (p < 0.05). These differences were reversed by glutamine treatment (p < 0.05). We conclude that glutamine supplementation might alleviate atherosclerosis through downregulation of O-GlcNAc, glycolysis, oxidative stress and proinflammatory pathway.

2023-05-15 | PXD042305 |

Aging stress and ceramide dependent RNA sequencing expression profiling depends on ceramide synthase 6

Project description:The bioactive sphingolipid ceramide, generated by ceramide synthase, is an adaptive stress effector induced in response to various stress stimuli such as aging. In fact, ceramide synthase (CerS) was first discovered in yeast as a longevity assurance gene (LAG1), as the reduced ceramide generation consequent to LAG1 deletion increased longevity. There are six homologues of mammalian LAG1/ceramide synthases (CerS1-6) that generate ceramides with different fatty acid chain lengths with distinct functions and hydrophobicity. Ceramide can be metabolized by sphingosine kinase 1 or 2 for the generation of pro-survival sphingosine 1-phosphate (S1P), which enhances immune cell egress to the blood stream, activating immune function. Ceramide is known to induce mitophagy and cell death. Here we investigated the role of this sphingolipid pathway in immune function, specifically T cell functions, via high throughput expression profiling.

2021-01-01 | GSE148498 | GEO

Differential gene expression in human abdominal aortic aneurysm and atherosclerosis

Project description:The aim of this study was to assess the relative gene expression in human AAA and AOD. Genome-wide expression analysis of abdominal aortic aneurysm (AAA) and aortic occlusive disease (AOD) specimens obtained from 20 patients with small AAA (mean maximum aortic diameter=54.3±2.3 mm), 29 patients with large AAA (mean maximum aortic diameter=68.4±14.3 mm), and 9 AOD patients (mean maximum aortic diameter=19.6±2.6 mm). Relative aortic gene expression was compared with that of 10 control aortic specimen of organ donors.

2015-06-30 | E-GEOD-57691 | biostudies-arrayexpress

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