Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1028798 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:ARRDC4 knock out Mouse Heart Analysis
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Project description:Purpose: Histone demethylase Kdm1a affected mouse SCLC progression through many down-stream factors or pathways. RNA-seq of mouse SCLC cell lines with or without Kdm1a knock-out was used to find the most enriched pathways in Kdm1a knock-out cells Methods: Total mRNA profiles of control (Crispr-V2) or Kdm1a knock-out mouse SCLC cell lines (Kdm1a-sg1, Kdm1a-sg2) were generated by deep sequencing, using Illumina Hiseq platform and 125 bp/150 bp paired-end reads. Index of the reference genome was built using Bowtie v2.2.3 and paired-end clean reads were aligned to the reference genome using TopHat v2.0.12. qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the mouse genome (build mm9). Commonly differential expression after Kdm1a knock-out with two different single guide RNAs to control mouse SCLC cells were selected with a fold change ≥1.5. Conclusions: Our data showed four Rest related pathways, including negative cell proliferation, negative cell differentiation, positive regulation of programmed cell death enriched, in commonly up-regulated genes after Kdm1a knock-out with twe different single guide RNAs. Meanwhile, the commonly down-regulated genes were mostly enriched in neron related pathways. These result illustrated the Kdm1a depletion inhibited mouse SCLC progression, defected its neuroendocrine phenotype through the up-regulation of Rest.
2022-01-20 | GSE125448 | GEO
Project description:Differential expression of mRNA in Tax1BP1 knock-out mice [mRNA]
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Project description:Many microRNAs (miRNAs) exist alongside abundant miRNA isoforms (isomiRs), most of which arise from post-maturation sequence modifications, such as 3’ uridylation and adenylation. However, the ways in which these sequence modifications affect miRNA function remain poorly understood. To this aim, we have generated single knock-out cell lines of TUT4, TUT7 and TENT2 (TUT2), as well as double knock-out (DKO) and triple knock-out (TKO) cell lines. Here, using these different cell lines, we have discovered that some of the redundant functions and specific functions of each tailing enzyme. Our study provides a comprehensive characterization of tailing on miRNAs.
2022-08-15 | GSE183384 | GEO
Project description:RNA-sequence of epithelial-specific Yap conditional knock out mouse lung
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Project description:Mice and other rodents typically used for nerve agent research have high concentrations of carboxylesterase in the blood that can act as natural scavengers for nerve agents. To develop an improved rodent system for the study of chemical warfare nerve agents, a serum carboxylesterase knock out mouse model was made (Es-1 knock out). This study utilized mRNA analysis to evaluate the effects of knocking out the serum carboxylesterase in a mouse model.
2018-05-04 | GSE114024 | GEO
Project description:Microarray of wild type, Shp2 knock-out, Pten knock-out and double knock-out erythroblasts
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