Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA102963 | ENA |
REPOSITORIES: ENA
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2007-10-20 | GSE9309 | GEO
Project description:Recovery of biological information (i.e. Breast tumor versus non-tumor part of breast) from microarray data under heterogeneous experimental conditions (Buffer1 versus Buffer2) using subgroup standardization. Microarray is a useful tool for gene expression analysis and prediction. For a given disease, a microarray database may come from various sources with different experimental setups, collected over time. This heterogeneity provides unnecessary complication in data analysis and, even worse, given false classification in clustering. Therefore, it is practically important to provide a standard data treatment for microarray data from heterogeneous experimental conditions. In this work, âsubgroup standardizationâ is proposed to compensate technical heterogeneities (e.g., buffers, time, machines etc.) in microarray experimental conditions. Provided with repetitive microarray experiments, over time and buffers, the results indicate that the proposed approach can extract correct biological information in the presence of technical irregularities. Hierarchical clustering is used to validate the effectiveness of the proposed approach. Experiment Overall Design: 98 of breast cancer specimens and 8 of non-tumor part of breast specimens were applied in the study. All the signals from the mRNA profile of each sample in the microarrays were normalized using the internal control RNA- Stratagene's human common reference RNA.
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2013-07-22 | GSE36600 | GEO
Project description:The data provide information of Gcn5 enrichment, H3K18 and H4K16 acetylation level and Histone H3 density for 5 different physioloigcal conditions during stress adpatation and stress recovery (normal growth, during stress adaptation, after stress adaptation, under stress recovery, after stress recovery) in yeast. The purpose of the study is to understand how histone acetyltransferase HATs (Gcn5) apply it is function in gene regulation by changing global or local histone acetylation level under different physiological conditions. Gcn5 enrichment, H3K18 and H4K16 acetylation level and Histone H3 density are mearured and compared to input signal for 5 different physioloigcal conditions (normal growth, during stress adaptation, after stress adaptation, under stress recovery, after stress recovery). Replicates are used.
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