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Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain - Study 2

ABSTRACT: Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain - Study 2

PROVIDER: PRJNA1031531 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26495003.fastq.gz	Fastqsanger.gz
	SRR26495004.fastq.gz	Fastqsanger.gz
	SRR26495005.fastq.gz	Fastqsanger.gz
	SRR26495006.fastq.gz	Fastqsanger.gz
	SRR26495007.fastq.gz	Fastqsanger.gz

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Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain

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Project description:In order to search for pharmacodynamic biomarkers of a TREM2 agonist antibody (Para09) in primates we performed bulk RNA-Seq on cortical tissue from cynomolgus monkeys after single IV injected doses. Changes in proliferation genes such as MKI67 were observed, as well as genes associated with glial activation, such as CHI3L1. This experiment demonstrates that our antibody induces transcriptional changes in the brain after peripheral administration.

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Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain [single nuclei RNA sequencing]

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Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain - Study 2

Project description:Monkeys were treated with a single i.v 200 mg/kg dose of hPara.09 or Anti-gD (Isotype control). Brain was harvested on days 4 and 28, and RNA was extracted from the cerebral cortex. The following groups are included: Group 1: Day 4 gD 200mg/kg n=6; Group 2: Day 4 hPara.09 200 mg/kg n=6; Group 3: Day 28 gD 200mg/kg n= 6; Group 4: Day 28 hPara.09 200 mg/kg n=6 (24 animals total). To assess expression heterogeneity between brain samples, 2 different frontal cortex punches were taken from each monkey, resulting in 48 total samples.

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